The Calcineurin-Drp1-Mediated Mitochondrial Fragmentation Is Aligned with the Differentiation of c-Kit Cardiac Progenitor Cells

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	The Calcineurin-Drp1-Mediated Mitochondrial Fragmentation Is Aligned with the Differentiation of c-Kit Cardiac Progenitor Cells
	Rahman, A; Li, YH; Ismail, NI; Chan, TK; Li, YZ; Xu, DC; Zhou, H; Ong, SB
	2023
发表期刊	INTERNATIONAL JOURNAL OF STEM CELLS
ISSN	2005-3606
卷号	16 期号:2 页码:123-134
摘要	Background and Objectives: The heart contains a pool of c-kit(+) progenitor cells which is believed to be able to regenerate. The differentiation of these progenitor cells is reliant on different physiological cues. Unraveling the underlying signals to direct differentiation of progenitor cells will be beneficial in controlling progenitor cell fate. In this regard, the role of the mitochondria in mediating cardiac progenitor cell fate remains unclear. Specifically, the association between changes in mitochondrial morphology with the differentiation status of c-kit(+) CPCs remains elusive. In this study, we investigated the relationship between mitochondrial morphology and the differentiation status of c-kit+ progenitor cells. Methods and Results: c-kit+ CPCs were isolated from 2-month-old male wild-type FVB mice. To activate differentiation, CPCs were incubated in alpha-minimal essential medium containing 10 nM dexamethasone for up to 7 days. To inhibit Drp1-mediated mitochondrial fragmentation, either 10 mu M or 50 mu M mdivi-1 was administered once at Day 0 and again at Day 2 of differentiation. To inhibit calcineurin, either 1 mu M or 5 mu M ciclosporin-A (CsA) was administered once at Day 0 and again at Day 2 of differentiation. Dexamethasone-induced differentiation of c-kit(+) progenitor cells is aligned with fragmentation of the mitochondria via a calcineurin-Drp1 pathway. Pharmacologically inhibiting mitochondrial fragmentation retains the undifferentiated state of the c-kit(+) progenitor cells. Conclusions: The findings from this study provide an alternative view of the role of mitochondrial fusion-fission in the differentiation of cardiac progenitor cells and the potential of pharmacologically manipulating the mitochondria to direct progenitor cell fate.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14123
专题	其他
推荐引用方式 GB/T 7714	Rahman, A,Li, YH,Ismail, NI,et al. The Calcineurin-Drp1-Mediated Mitochondrial Fragmentation Is Aligned with the Differentiation of c-Kit Cardiac Progenitor Cells[J]. INTERNATIONAL JOURNAL OF STEM CELLS,2023,16(2):123-134.
APA	Rahman, A.,Li, YH.,Ismail, NI.,Chan, TK.,Li, YZ.,...&Ong, SB.(2023).The Calcineurin-Drp1-Mediated Mitochondrial Fragmentation Is Aligned with the Differentiation of c-Kit Cardiac Progenitor Cells.INTERNATIONAL JOURNAL OF STEM CELLS,16(2),123-134.
MLA	Rahman, A,et al."The Calcineurin-Drp1-Mediated Mitochondrial Fragmentation Is Aligned with the Differentiation of c-Kit Cardiac Progenitor Cells".INTERNATIONAL JOURNAL OF STEM CELLS 16.2(2023):123-134.

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2024061118.pdf（2957KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文