Vertebrate OTOP1 is also an alkali-activated channel

	Vertebrate OTOP1 is also an alkali-activated channel
	Tian, LF; Zhang, H; Yang, SL; Luo, AN; Kamau, PM; Hu, JM; Luo, L; Lai, R
	2023
发表期刊	NATURE COMMUNICATIONS
卷号	14 期号:1
摘要	Although alkaline sensation is critical for survival, alkali-activated receptors are yet to be identified in vertebrates. Here, we showed that the OTOP1 channel can be directly activated by extracellular alkali. Notably, OTOP1 biphasically mediated proton influx and efflux with extracellular acid and base stimulation, respectively. Mutations of K221 and R554 at the S5-S6 and S11-S12 linkers significantly reduced alkali affinity without affecting acid activation, suggesting that different domains are responsible for acid- and alkali-activation of OTOP1. The selectivity for H+ was significantly higher in OTOP1 activated by alkali than that by acid, further suggesting that the two activations might be independent gating processes. Given that the alkali-activation of OTOP1 and the required key residues were conserved in the six representative vertebrates, we cautiously propose that OTOP1 participates in alkaline sensation in vertebrates. Thus, our study identified OTOP1 as an alkali-activated channel. pH sensation is critical for survival of vertebrates. Here, authors found six vertebrate OTOP1 channels that were highly conserved and directly activated by extracellular alkali. Key mutations of OTOP1 reduced alkali affinity without affecting acid activation.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14155
专题	科研部门_天然药物功能蛋白质学科组（赖仞）
推荐引用方式 GB/T 7714	Tian, LF,Zhang, H,Yang, SL,et al. Vertebrate OTOP1 is also an alkali-activated channel[J]. NATURE COMMUNICATIONS,2023,14(1).
APA	Tian, LF.,Zhang, H.,Yang, SL.,Luo, AN.,Kamau, PM.,...&Lai, R.(2023).Vertebrate OTOP1 is also an alkali-activated channel.NATURE COMMUNICATIONS,14(1).
MLA	Tian, LF,et al."Vertebrate OTOP1 is also an alkali-activated channel".NATURE COMMUNICATIONS 14.1(2023).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2024061327.pdf（3017KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文