SARS-CoV-2 NSP12 utilizes various host splicing factors for replication and splicing regulation

	SARS-CoV-2 NSP12 utilizes various host splicing factors for replication and splicing regulation
	Yang, L; Zeng, XT; Luo, RH; Ren, SX; Liang, LL; Huang, QX; Tang, Y; Fan, H; Ren, HY; Zhang, WJ; Zheng, YT; Cheng, W
	2024
发表期刊	JOURNAL OF MEDICAL VIROLOGY
ISSN	0146-6615
卷号	96 期号:1
摘要	The RNA-dependent RNA polymerase (RdRp) is a crucial element in the replication and transcription of RNA viruses. Although the RdRps of lethal human coronaviruses severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), SARS-CoV, and Middle East respiratory syndrome coronavirus (MERS-CoV) have been extensively studied, the molecular mechanism of the catalytic subunit NSP12, which is involved in pathogenesis, remains unclear. In this study, the biochemical and cell biological results demonstrate the interactions between SARS-CoV-2 NSP12 and seven host proteins, including three splicing factors (SLU7, PPIL3, and AKAP8). The entry efficacy of SARS-CoV-2 considerably decreased when SLU7 or PPIL3 was knocked out, indicating that abnormal splicing of the host genome was responsible for this occurrence. Furthermore, the polymerase activity and stability of SARS-CoV-2 RdRp were affected by the three splicing factors to varying degrees. In addition, NSP12 and its homologues from SARS-CoV and MERS-CoV suppressed the alternative splicing of cellular genes, which were influenced by the three splicing factors. Overall, our research illustrates that SARS-CoV-2 NSP12 can engage with various splicing factors, thereby impacting virus entry, replication, and gene splicing. This not only improves our understanding of how viruses cause diseases but also lays the foundation for the development of antiviral therapies.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14203
专题	科研部门_分子免疫药理学（郑永唐）
推荐引用方式 GB/T 7714	Yang, L,Zeng, XT,Luo, RH,et al. SARS-CoV-2 NSP12 utilizes various host splicing factors for replication and splicing regulation[J]. JOURNAL OF MEDICAL VIROLOGY,2024,96(1).
APA	Yang, L.,Zeng, XT.,Luo, RH.,Ren, SX.,Liang, LL.,...&Cheng, W.(2024).SARS-CoV-2 NSP12 utilizes various host splicing factors for replication and splicing regulation.JOURNAL OF MEDICAL VIROLOGY,96(1).
MLA	Yang, L,et al."SARS-CoV-2 NSP12 utilizes various host splicing factors for replication and splicing regulation".JOURNAL OF MEDICAL VIROLOGY 96.1(2024).

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