Identification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, China

	Identification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, China
	Tang, Y; Zhao, K; Yin, HM; Yang, LP; Wu, YC; Li, FY; Yang, Z; Lu, HX; Wang, B; Yang, Y; Zhang, YZ; Yang, XL
	2024
发表期刊	VIRUSES-BASEL
ISSN	1999-4915
卷号	16 期号:6
摘要	Hepatitis A virus (HAV), a member of the genus Hepatovirus (Picornaviridae HepV), remains a significant viral pathogen, frequently causing enterically transmitted hepatitis worldwide. In this study, we conducted an epidemiological survey of HepVs carried by small terrestrial mammals in the wild in Yunnan Province, China. Utilizing HepV-specific broad-spectrum RT-PCR, next-generation sequencing (NGS), and QNome nanopore sequencing (QNS) techniques, we identified and characterized two novel HepVs provisionally named EpMa-HAV and EpLe-HAV, discovered in the long-tailed mountain shrew (Episoriculus macrurus) and long-tailed brown-toothed shrew (Episoriculus leucops), respectively. Our sequence and phylogenetic analyses of EpMa-HAV and EpLe-HAV indicated that they belong to the species Hepatovirus I (HepV-I) clade II, also known as the Chinese shrew HepV clade. Notably, the codon usage bias pattern of novel shrew HepVs is consistent with that of previously identified Chinese shrew HepV. Furthermore, our structural analysis demonstrated that shrew HepVs differ from other mammalian HepVs in RNA secondary structure and exhibit variances in key protein sites. Overall, the discovery of two novel HepVs in shrews expands the host range of HepV and underscores the existence of genetically diverse animal homologs of human HAV within the genus HepV.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14214
专题	科研部门_疫源动物病毒（杨兴娄）
推荐引用方式 GB/T 7714	Tang, Y,Zhao, K,Yin, HM,et al. Identification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, China[J]. VIRUSES-BASEL,2024,16(6).
APA	Tang, Y.,Zhao, K.,Yin, HM.,Yang, LP.,Wu, YC.,...&Yang, XL.(2024).Identification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, China.VIRUSES-BASEL,16(6).
MLA	Tang, Y,et al."Identification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, China".VIRUSES-BASEL 16.6(2024).

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