Establishment of a human organoid-based evaluation system for assessing interspecies infection risk of animal-borne coronaviruses

	Establishment of a human organoid-based evaluation system for assessing interspecies infection risk of animal-borne coronaviruses
	Gong, QC; Jiang, RD; Ji, LA; Lin, HF; Liu, MQ; Tang, XF; Yang, Y; Han, W; Chen, J; Guo, ZS; Wang, Q; Li, Q; Wang, X; Jiang, TT; Xie, SZ; Yang, XL; Zhou, P; Shi, ZL; Lin, XH
	2024
发表期刊	EMERGING MICROBES & INFECTIONS
ISSN	2222-1751
卷号	13 期号:1
摘要	The COVID-19 pandemic presents a major threat to global public health. Several lines of evidence have shown that the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), along with two other highly pathogenic coronaviruses, SARS-CoV and Middle East Respiratory Syndrome (MERS-CoV) originated from bats. To prevent and control future coronavirus outbreaks, it is necessary to investigate the interspecies infection and pathogenicity risks of animal-related coronaviruses. Currently used infection models, including in vitro cell lines and in vivo animal models, fail to fully mimic the primary infection in human tissues. Here, we employed organoid technology as a promising new model for studying emerging pathogens and their pathogenic mechanisms. We investigated the key host-virus interaction patterns of five human coronaviruses (SARS-CoV-2 original strain, Omicron BA.1, MERS-CoV, HCoV-229E, and HCoV-OC43) in different human respiratory organoids. Five indicators, including cell tropism, invasion preference, replication activity, host response and virus-induced cell death, were developed to establish a comprehensive evaluation system to predict coronavirus interspecies infection and pathogenicity risks. Using this system, we further examined the pathogenicity and interspecies infection risks of three SARS-related coronaviruses (SARSr-CoV), including WIV1 and rRsSHC014S from bats, and MpCoV-GX from pangolins. Moreover, we found that cannabidiol, a non-psychoactive plant extract, exhibits significant inhibitory effects on various coronaviruses in human lung organoid. Cannabidiol significantly enhanced interferon-stimulated gene expression but reduced levels of inflammatory cytokines. In summary, our study established a reliable comprehensive evaluation system to analyse infection and pathogenicity patterns of zoonotic coronaviruses, which could aid in prevention and control of potentially emerging coronavirus diseases.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14216
专题	科研部门_疫源动物病毒（杨兴娄）
推荐引用方式 GB/T 7714	Gong, QC,Jiang, RD,Ji, LA,et al. Establishment of a human organoid-based evaluation system for assessing interspecies infection risk of animal-borne coronaviruses[J]. EMERGING MICROBES & INFECTIONS,2024,13(1).
APA	Gong, QC.,Jiang, RD.,Ji, LA.,Lin, HF.,Liu, MQ.,...&Lin, XH.(2024).Establishment of a human organoid-based evaluation system for assessing interspecies infection risk of animal-borne coronaviruses.EMERGING MICROBES & INFECTIONS,13(1).
MLA	Gong, QC,et al."Establishment of a human organoid-based evaluation system for assessing interspecies infection risk of animal-borne coronaviruses".EMERGING MICROBES & INFECTIONS 13.1(2024).

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2024073155.pdf（4891KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文