Human transferrin receptor can mediate SARS-CoV-2 infection

	Human transferrin receptor can mediate SARS-CoV-2 infection
	Liao, ZY; Wang, CM; Tang, XP; Yang, ML; Duan, ZL; Liu, L; Lu, SY; Ma, L; Cheng, RM; Wang, G; Liu, HQ; Yang, S; Xu, JW; Tadese, DA; Mwangi, J; Kamau, PM; Zhang, ZY; Yang, L; Liao, GY; Zhao, XD; Peng, XZ; Lai, R
	2024
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
卷号	121 期号:10
摘要	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been detected in almost all organs of coronavirus disease-19 patients, although some organs do not express angiotensin-converting enzyme-2 (ACE2), a known receptor of SARS-CoV-2, implying the presence of alternative receptors and/or co-receptors. Here, we show that the ubiquitously distributed human transferrin receptor (TfR), which binds to diferric transferrin to traffic between membrane and endosome for the iron delivery cycle, can ACE2-independently mediate SARS-CoV-2 infection. Human, not mouse TfR, interacts with Spike protein with a high affinity (K-D similar to 2.95 nM) to mediate SARS-CoV-2 endocytosis. TfR knock-down (TfR-deficiency is lethal) and overexpression inhibit and promote SARS-CoV-2 infection, respectively. Humanized TfR expression enables SARS-CoV-2 infection in baby hamster kidney cells and C57 mice, which are known to be insusceptible to the virus infection. Soluble TfR, Tf, designed peptides blocking TfR-Spike interaction and anti-TfR antibody show significant anti-COVID-19 effects in cell and monkey models. Collectively, this report indicates that TfR is a receptor/co-receptor of SARS-CoV-2 mediating SARS-CoV-2 entry and infectivity by likely using the TfR trafficking pathway.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14222
专题	科研部门_天然药物功能蛋白质学科组（赖仞）
推荐引用方式 GB/T 7714	Liao, ZY,Wang, CM,Tang, XP,et al. Human transferrin receptor can mediate SARS-CoV-2 infection[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2024,121(10).
APA	Liao, ZY.,Wang, CM.,Tang, XP.,Yang, ML.,Duan, ZL.,...&Lai, R.(2024).Human transferrin receptor can mediate SARS-CoV-2 infection.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,121(10).
MLA	Liao, ZY,et al."Human transferrin receptor can mediate SARS-CoV-2 infection".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 121.10(2024).

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2024073156.pdf（2970KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文