A rationally designed fluorescence probe achieves highly specific and long-term detection of senescence in vitro and in vivo

	A rationally designed fluorescence probe achieves highly specific and long-term detection of senescence in vitro and in vivo
	Hu, L; Dong, CJ; Wang, Z; He, SY; Yang, YW; Zi, MT; Li, HQ; Zhang, YH; Chen, CJ; Zheng, RZ; Jia, ST; Liu, J; Zhang, X; He, YH
	2023
发表期刊	AGING CELL
ISSN	1474-9718
卷号	22 期号:8
摘要	Senescent cells (SnCs) are implicated in aging and various age-related pathologies. Targeting SnCs can treat age-related diseases and extend health span. However, precisely tracking and visualizing of SnCs is still challenging, especially in in vivo environments. Here, we developed a near-infrared (NIR) fluorescent probe (XZ1208) that targets beta-galactosidase (beta-Gal), a well-accepted biomarker for cellular senescence. XZ1208 can be cleaved rapidly by beta-Gal and produces a strong fluorescence signal in SnCs. We demonstrated the high specificity and sensitivity of XZ1208 in labeling SnCs in naturally aged, total body irradiated (TBI), and progeroid mouse models. XZ1208 achieved a long-term duration of over 6 days in labeling senescence without causing significant toxicities and accurately detected the senolytic effects of ABT263 on eliminating SnCs. Furthermore, XZ1208 was applied to monitor SnCs accumulated in fibrotic diseases and skin wound healing models. Overall, we developed a tissue-infiltrating NIR probe and demonstrated its excellent performance in labeling SnCs in aging and senescence-associated disease models, indicating great potential for application in aging studies and diagnosis of senescence-associated diseases.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14352
专题	科研部门_衰老调控与功能研究（何永捍）
推荐引用方式 GB/T 7714	Hu, L,Dong, CJ,Wang, Z,et al. A rationally designed fluorescence probe achieves highly specific and long-term detection of senescence in vitro and in vivo[J]. AGING CELL,2023,22(8).
APA	Hu, L.,Dong, CJ.,Wang, Z.,He, SY.,Yang, YW.,...&He, YH.(2023).A rationally designed fluorescence probe achieves highly specific and long-term detection of senescence in vitro and in vivo.AGING CELL,22(8).
MLA	Hu, L,et al."A rationally designed fluorescence probe achieves highly specific and long-term detection of senescence in vitro and in vivo".AGING CELL 22.8(2023).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2024053110.pdf（4512KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文