Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae

	Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae
	Cheng, L; Zhao, SJ; Li, TY; Hou, S; Luo, ZQ; Xu, JS; Yu, WF; Jiang, SY; Monti, M; Schindler, D; Zhang, WM; Hou, CH; Ma, YX; Cai, YZ; Boeke, JD; Dai, JB
	2024
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	15 期号:1
摘要	Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) is a promising tool to study genomic rearrangements. However, the potential of SCRaMbLE to study genomic rearrangements is currently hindered, because a strain containing all 16 synthetic chromosomes is not yet available. Here, we construct SparLox83R, a yeast strain containing 83 loxPsym sites distributed across all 16 chromosomes. SCRaMbLE of SparLox83R produces versatile genome-wide genomic rearrangements, including inter-chromosomal events. Moreover, when combined with synthetic chromosomes, SCRaMbLE of hetero-diploids with SparLox83R leads to increased diversity of genomic rearrangements and relatively faster evolution of traits compared to hetero-diploids only with wild-type chromosomes. Analysis of the SCRaMbLEd strain with increased tolerance to nocodazole demonstrates that genomic rearrangements can perturb the transcriptome and 3D genome structure and consequently impact phenotypes. In summary, a genome with sparsely distributed loxPsym sites can serve as a powerful tool for studying the consequence of genomic rearrangements and accelerating strain engineering in Saccharomyces cerevisiae.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14357
专题	科研部门_进化发育与四维基因调控（候春晖）
推荐引用方式 GB/T 7714	Cheng, L,Zhao, SJ,Li, TY,et al. Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae[J]. NATURE COMMUNICATIONS,2024,15(1).
APA	Cheng, L.,Zhao, SJ.,Li, TY.,Hou, S.,Luo, ZQ.,...&Dai, JB.(2024).Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae.NATURE COMMUNICATIONS,15(1).
MLA	Cheng, L,et al."Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae".NATURE COMMUNICATIONS 15.1(2024).

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