High-throughput Pore-C reveals the single-allele topology and cell type-specificity of 3D genome folding

	High-throughput Pore-C reveals the single-allele topology and cell type-specificity of 3D genome folding
	Zhong, JY; Niu, LJ; Lin, ZB; Bai, X; Chen, Y; Luo, F; Hou, CH; Xiao, CL
	2023
发表期刊	NATURE COMMUNICATIONS
卷号	14 期号:1
摘要	Canonical three-dimensional (3D) genome structures represent the ensemble average of pairwise chromatin interactions but not the single-allele topologies in populations of cells. Recently developed Pore-C can capture multiway chromatin contacts that reflect regional topologies of single chromosomes. By carrying out high-throughput Pore-C, we reveal extensive but regionally restricted clusters of single-allele topologies that aggregate into canonical 3D genome structures in two human cell types. We show that fragments in multi-contact reads generally coexist in the same TAD. In contrast, a concurrent significant proportion of multi-contact reads span multiple compartments of the same chromatin type over megabase distances. Synergistic chromatin looping between multiple sites in multi-contact reads is rare compared to pairwise interactions. Interestingly, the single-allele topology clusters are cell type-specific even inside highly conserved TADs in different types of cells. In summary, HiPore-C enables global characterization of single-allele topologies at an unprecedented depth to reveal elusive genome folding principles. Here the authors establish a high throughput Pore-C (HiPore-C) method increasing the sequencing output of multi-way chromatin contacts and reveal single-allele topology diversity and cell type-specificity of 3D genome folding.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14363
专题	科研部门_进化发育与四维基因调控（候春晖）
推荐引用方式 GB/T 7714	Zhong, JY,Niu, LJ,Lin, ZB,et al. High-throughput Pore-C reveals the single-allele topology and cell type-specificity of 3D genome folding[J]. NATURE COMMUNICATIONS,2023,14(1).
APA	Zhong, JY.,Niu, LJ.,Lin, ZB.,Bai, X.,Chen, Y.,...&Xiao, CL.(2023).High-throughput Pore-C reveals the single-allele topology and cell type-specificity of 3D genome folding.NATURE COMMUNICATIONS,14(1).
MLA	Zhong, JY,et al."High-throughput Pore-C reveals the single-allele topology and cell type-specificity of 3D genome folding".NATURE COMMUNICATIONS 14.1(2023).

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