RNAi efficiency is enhanced through knockdown of double-stranded RNA-degrading enzymes in butterfly Papilio xuthus

KIZ OpenIR

	RNAi efficiency is enhanced through knockdown of double-stranded RNA-degrading enzymes in butterfly Papilio xuthus
	Shu, Q; Liu, GC; He, JW; Hu, P; Dong, ZW; Zhao, RP; Zhang, HR; Li, XY
	2024
发表期刊	ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
ISSN	0739-4462
卷号	115 期号:4
摘要	The efficiency of RNA interference (RNAi) has always limited the research on the phenotype innovation of Lepidoptera insects. Previous studies have found that double-stranded RNA-degrading enzyme (dsRNase) is an important factor in RNAi efficiency, but there have been no relevant reports in butterflies (Papilionoidea). Papilio xuthus is one of the important models in butterflies with an extensive experimental application value. To explore the effect of dsRNase in the RNAi efficiency on butterflies, six dsRNase genes (PxdsRNase 1-6) were identified in P. xuthus genome, and their dsRNA-degrading activities were subsequently detected by ex vivo assays. The result shows that the dsRNA-degrading ability of gut content (<1 h) was higher than hemolymph content (>12 h). We then investigated the expression patterns of these PxdsRNase genes during different tissues and developmental stages, and related RNAi experiments were carried out. Our results show that different PxdsRNase genes had different expression levels at different developmental stages and tissues. The expression of PxdsRNase2, PxdsRNase3, and PxdsRNase6 were upregulated significantly through dsGFP injection, and PxdsRNase genes can be silenced effectively by injecting their corresponding dsRNA. RNAi-of-RNAi studies with PxEbony, which acts as a reporter gene, observed that silencing PxdsRNase genes can increase RNAi efficiency significantly. These results confirm that silencing dsRNase genes can improve RNAi efficiency in P. xuthus significantly, providing a reference for the functional study of insects such as butterflies with low RNAi efficiency.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14373
专题	昆明动物研究所
推荐引用方式 GB/T 7714	Shu, Q,Liu, GC,He, JW,et al. RNAi efficiency is enhanced through knockdown of double-stranded RNA-degrading enzymes in butterfly Papilio xuthus[J]. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,2024,115(4).
APA	Shu, Q.,Liu, GC.,He, JW.,Hu, P.,Dong, ZW.,...&Li, XY.(2024).RNAi efficiency is enhanced through knockdown of double-stranded RNA-degrading enzymes in butterfly Papilio xuthus.ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,115(4).
MLA	Shu, Q,et al."RNAi efficiency is enhanced through knockdown of double-stranded RNA-degrading enzymes in butterfly Papilio xuthus".ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 115.4(2024).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2024073006.pdf（2520KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文