Cloning and characterization of luciferase from an Asian firefly Pygoluciola qingyu and its comparison with other beetle luciferases

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	Cloning and characterization of luciferase from an Asian firefly Pygoluciola qingyu and its comparison with other beetle luciferases
	Li, J; Liu, W; Liu, GC; Dong, ZW; He, JW; Zhao, RP; Wang, W; Li, XY
	2024
发表期刊	PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
ISSN	1474-905X
卷号	23 期号:4 页码:719-729
摘要	The bioluminescence system of luminescent beetles has extensive applications in biological imaging, protein labeling and drug screening. To explore wild luciferases with excellent catalytic activity and thermal stability, we cloned the luciferase of Pygoluciola qingyu, one species living in areas of high temperature and with strong bioluminescence, by combining transcriptomic sequencing and reverse transcription polymerase chain reaction (RT-PCR). The total length of luciferase gene is 1638 bp and the luciferase consists 544 amino acids. The recombinant P. qingyu luciferase was produced in vitro and its characteristics were compared with those of eight luciferases from China firefly species and two commercial luciferases. Compared with these luciferases, the P. qingyu luciferase shows the highest luminescence activity at room temperature (about 25-28 celcius) with similar KM value for d-luciferin and ATP to the Photinus pyralis luciferase. The P. qingyu luciferase activity was highest at 35 degrees celcius and can keep high activity at 30-40 degrees celcius, which suggests the potential of P. qingyu luciferase for in vivo and cell application. Our results provide new insights into P. qingyu luciferase and give a new resource for the application of luciferases.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14374
专题	昆明动物研究所
推荐引用方式 GB/T 7714	Li, J,Liu, W,Liu, GC,et al. Cloning and characterization of luciferase from an Asian firefly Pygoluciola qingyu and its comparison with other beetle luciferases[J]. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES,2024,23(4):719-729.
APA	Li, J.,Liu, W.,Liu, GC.,Dong, ZW.,He, JW.,...&Li, XY.(2024).Cloning and characterization of luciferase from an Asian firefly Pygoluciola qingyu and its comparison with other beetle luciferases.PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES,23(4),719-729.
MLA	Li, J,et al."Cloning and characterization of luciferase from an Asian firefly Pygoluciola qingyu and its comparison with other beetle luciferases".PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES 23.4(2024):719-729.

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2024073018.pdf（4595KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文