LET-767 determines lipid droplet protein targeting and lipid homeostasis

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	LET-767 determines lipid droplet protein targeting and lipid homeostasis
	Fu, L; Zhang, JJ; Wang, YL; Wu, HY; Xu, XM; Li, CX; Li, JR; Liu, J; Wang, HZ; Jiang, X; Li, ZH; He, YM; Liu, PS; Wu, YJ; Zou, XJ; Liang, B
	2024
发表期刊	JOURNAL OF CELL BIOLOGY
ISSN	0021-9525
卷号	223 期号:6
摘要	LET-767, a member of hydroxysteroid dehydrogenase, interacts with ADP-ribosylation factor 1 (ARF-1) to prevent ARF-1 lipid droplet (LD) translocation for appropriate LD protein targeting. Deficiency of LET-767 triggers ARF-1 and ATGL-1 (adipose triglyceride lipase) LD translocation, leading to displacement of LD proteins and promoted lipolysis. Lipid droplets (LDs) are composed of a core of neutral lipids wrapped by a phospholipid (PL) monolayer containing several hundred proteins that vary between different cells or organisms. How LD proteins target to LDs is still largely unknown. Here, we show that RNAi knockdown or gene mutation of let-767, encoding a member of hydroxysteroid dehydrogenase (HSD), displaced the LD localization of three well-known LD proteins: DHS-3 (dehydrogenase/reductase), PLIN-1 (perilipin), and DGAT-2 (diacylglycerol O-acyltransferase 2), and also prevented LD growth in Caenorhabditis elegans. LET-767 interacts with ARF-1 (ADP-ribosylation factor 1) to prevent ARF-1 LD translocation for appropriate LD protein targeting and lipid homeostasis. Deficiency of LET-767 leads to the release of ARF-1, which further recruits and promotes translocation of ATGL-1 (adipose triglyceride lipase) to LDs for lipolysis. The displacement of LD proteins caused by LET-767 deficiency could be reversed by inhibition of either ARF-1 or ATGL-1. Our work uncovers a unique LET-767 for determining LD protein targeting and maintaining lipid homeostasis.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14424
专题	昆明动物研究所
推荐引用方式 GB/T 7714	Fu, L,Zhang, JJ,Wang, YL,et al. LET-767 determines lipid droplet protein targeting and lipid homeostasis[J]. JOURNAL OF CELL BIOLOGY,2024,223(6).
APA	Fu, L.,Zhang, JJ.,Wang, YL.,Wu, HY.,Xu, XM.,...&Liang, B.(2024).LET-767 determines lipid droplet protein targeting and lipid homeostasis.JOURNAL OF CELL BIOLOGY,223(6).
MLA	Fu, L,et al."LET-767 determines lipid droplet protein targeting and lipid homeostasis".JOURNAL OF CELL BIOLOGY 223.6(2024).

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