Ptip safeguards the epigenetic control of skeletal stem cell quiescence and potency in skeletogenesis

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	Ptip safeguards the epigenetic control of skeletal stem cell quiescence and potency in skeletogenesis
	Liang, JF; Wang, J; Sui, BD; Tong, YB; Chai, JH; Zhou, Q; Zheng, CX; Wang, H; Kong, L; Zhang, HJ; Bai, Y
	2024
发表期刊	SCIENCE BULLETIN
ISSN	2095-9273
卷号	69 期号:13 页码:2099-2113
摘要	Stem cells remain in a quiescent state for long-term maintenance and preservation of potency; this process requires fine-tuning regulatory mechanisms. In this study, we identified the epigenetic landscape along the developmental trajectory of skeletal stem cells (SSCs) in skeletogenesis governed by a key regulator, Ptip (also known as Paxip1, Pax interaction with transcription-activation domain protein-1). Our results showed that Ptip is required for maintaining the quiescence and potency of SSCs, and loss of Ptip in type II collagen (Col2) + progenitors causes abnormal activation and differentiation of SSCs, impaired growth plate morphogenesis, and long bone dysplasia. We also found that Ptip suppressed the glycolysis of SSCs through downregulation of phosphoglycerate kinase 1 ( Pgk1 ) by repressing histone H3 lysine 27 acetylation (H3K27ac) at the promoter region. Notably, inhibition of glycolysis improved the function of SSCs despite Ptip deficiency. To the best of our knowledge, this is the first study to establish an epigenetic framework based on Ptip, which safeguards skeletal stem cell quiescence and potency through metabolic control. This framework is expected to improve SSC-based treatments of bone developmental disorders. (c) 2024 Science China Press. Published by Elsevier B.V. and Science China Press. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14432
专题	昆明动物研究所
推荐引用方式 GB/T 7714	Liang, JF,Wang, J,Sui, BD,et al. Ptip safeguards the epigenetic control of skeletal stem cell quiescence and potency in skeletogenesis[J]. SCIENCE BULLETIN,2024,69(13):2099-2113.
APA	Liang, JF.,Wang, J.,Sui, BD.,Tong, YB.,Chai, JH.,...&Bai, Y.(2024).Ptip safeguards the epigenetic control of skeletal stem cell quiescence and potency in skeletogenesis.SCIENCE BULLETIN,69(13),2099-2113.
MLA	Liang, JF,et al."Ptip safeguards the epigenetic control of skeletal stem cell quiescence and potency in skeletogenesis".SCIENCE BULLETIN 69.13(2024):2099-2113.

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2024080123.pdf（6888KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文