PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens

	PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens
	Kinoshita, K; Tanabe, K; Nakamura, Y; Nishijima, KI; Suzuki, T; Okuzaki, Y; Mizushima, S; Wang, MS; Khan, SU; Xu, KX; Jamal, MA; Wei, TY; Zhao, H; Su, YH; Sun, FZ; Liu, G; Zhu, FX; Zhao, HY; Wei, HJ
	2024
发表期刊	COMMUNICATIONS BIOLOGY
卷号	7 期号:1
摘要	Primordial germ cells (PGCs) are vital for producing sperm and eggs and are crucial for conserving chicken germplasm and creating genetically modified chickens. However, efforts to use PGCs for preserving native chicken germplasm and genetic modification via CRISPR/Cas9 are limited. Here we show that we established 289 PGC lines from eight Chinese chicken populations with an 81.6% success rate. We regenerated Piao chickens by repropagating cryopreserved PGCs and transplanting them into recipient chickens, achieving a 12.7% efficiency rate. These regenerated chickens carried mitochondrial DNA from female donor PGC and the rumplessness mutation from both male and female donors. Additionally, we created the TYRP1 (tyrosinase-related protein 1) knockout (KO) PGC lines via CRISPR/Cas9. Transplanting KO cells into male recipients and mating them with wild-type hens produced four TYRP1 KO chickens with brown plumage due to reduced eumelanin production. Our work demonstrates efficient PGC culture, cryopreservation, regeneration, and gene editing in chickens.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14457
专题	科研部门_进化与生物多样性基因组学（王明山）
推荐引用方式 GB/T 7714	Kinoshita, K,Tanabe, K,Nakamura, Y,et al. PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens[J]. COMMUNICATIONS BIOLOGY,2024,7(1).
APA	Kinoshita, K.,Tanabe, K.,Nakamura, Y.,Nishijima, KI.,Suzuki, T.,...&Wei, HJ.(2024).PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens.COMMUNICATIONS BIOLOGY,7(1).
MLA	Kinoshita, K,et al."PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens".COMMUNICATIONS BIOLOGY 7.1(2024).

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