Development of a Mengla virus minigenome and comparison of its polymerase complexes with those of other filoviruses

	Development of a Mengla virus minigenome and comparison of its polymerase complexes with those of other filoviruses
	Xie, SZ; Yao, K; Li, B; Peng, C; Yang, XL; Shi, ZL
	2024
发表期刊	VIROLOGICA SINICA
ISSN	1674-0769
卷号	39 期号:3 页码:459-468
摘要	Ebola virus (EBOV) and Marburg virus (MARV), members of the Filoviridae family, are highly pathogenic and can cause hemorrhagic fevers, significantly impacting human society. Bats are considered reservoirs of these viruses because related filoviruses have been discovered in bats. However, due to the requirement for maximum containment laboratories when studying infectious viruses, the characterization of bat filoviruses often relies on pseudoviruses and minigenome systems. In this study, we used RACE technology to sequence the 3'-leader and 5'-trailer of Mengla virus (MLAV) and constructed a minigenome. Similar to MARV, the transcription activities of the MLAV minigenome are independent of VP30. We further assessed the effects of polymorphisms at the 5' end on MLAV minigenome activity and identified certain mutations that decrease minigenome reporter efficiency, probably due to alterations in the RNA secondary structure. The reporter activity upon recombination of the 3'-leaders and 5'-trailers of MLAV, MARV, and EBOV with those of the homologous or heterologous minigenomes was compared and it was found that the polymerase complex and leader and trailer sequences exhibit intrinsic specificities. Additionally, we investigated whether the polymerase complex proteins from EBOV and MARV support MLAV minigenome RNA synthesis and found that the homologous system is more efficient than the heterologous system. Remdesivir efficiently inhibited MLAV as well as EBOV replication. In summary, this study provides new information on bat filoviruses and the minigenome will be a useful tool for high-throughput antiviral drug screening.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14465
专题	科研部门_疫源动物病毒（杨兴娄）
推荐引用方式 GB/T 7714	Xie, SZ,Yao, K,Li, B,et al. Development of a Mengla virus minigenome and comparison of its polymerase complexes with those of other filoviruses[J]. VIROLOGICA SINICA,2024,39(3):459-468.
APA	Xie, SZ,Yao, K,Li, B,Peng, C,Yang, XL,&Shi, ZL.(2024).Development of a Mengla virus minigenome and comparison of its polymerase complexes with those of other filoviruses.VIROLOGICA SINICA,39(3),459-468.
MLA	Xie, SZ,et al."Development of a Mengla virus minigenome and comparison of its polymerase complexes with those of other filoviruses".VIROLOGICA SINICA 39.3(2024):459-468.

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