Identification of novel rodent and shrew orthohepeviruses sheds light on hepatitis E virus evolution

	Identification of novel rodent and shrew orthohepeviruses sheds light on hepatitis E virus evolution
	Li, FL; Wang, B; Han, PY; Li, B; Si, HR; Zhu, Y; Yin, HM; Zong, LD; Tang, Y; Shi, ZL; Hu, B; Yang, XL; Zhang, YZ
	2025
发表期刊	ZOOL RES
ISSN	2095-8137
卷号	46 期号:1 页码:103-121
摘要	The family Hepeviridae has seen an explosive expansion in its host range in recent years, yet the evolutionary trajectory of this zoonotic pathogen remains largely unknown. The emergence of rat hepatitis E virus (HEV) has introduced a new public health threat due to its potential for zoonotic transmission. This study investigated 2 464 wild small mammals spanning four animal orders, eight families, 21 genera, and 37 species in Yunnan Province, China. Using broadly reactive reverse transcription-polymerase chain reaction (RT-PCR), we systematically screened the presence and prevalence of Orthohepevirus and identified 192 positive specimens from 10 species, corresponding to an overall detection rate of 7.79%. Next-generation sequencing enabled the recovery of 24 full-length genomic sequences from eight host species, including Bandicota bengalensis, Eothenomys eleusis, and Episoriculus caudatus, representing newly reported host species for Orthohepevirus strains. Phylogenetic and sequence analyses revealed extensive genetic diversity within orthohepeviruses infecting rodents and shrews. Notably, among the identified strains, 20 were classified as Rocahepevirus ratti C1, two as C3, and one as Rocahepevirus eothenomi, while the remaining strain exhibited significant divergence, precluding classification. Evolutionary analyses highlighted close associations between orthohepeviruses and their respective host taxa, with distinct phylogenetic clustering patterns observed across different host orders. These findings emphasize the critical roles of co-speciation and cross-species transmission in shaping the evolutionary trajectories of the genera Paslahepevirus and Rocahepevirus.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14466
专题	科研部门_疫源动物病毒（杨兴娄）
推荐引用方式 GB/T 7714	Li, FL,Wang, B,Han, PY,et al. Identification of novel rodent and shrew orthohepeviruses sheds light on hepatitis E virus evolution[J]. ZOOL RES,2025,46(1):103-121.
APA	Li, FL.,Wang, B.,Han, PY.,Li, B.,Si, HR.,...&Zhang, YZ.(2025).Identification of novel rodent and shrew orthohepeviruses sheds light on hepatitis E virus evolution.ZOOL RES,46(1),103-121.
MLA	Li, FL,et al."Identification of novel rodent and shrew orthohepeviruses sheds light on hepatitis E virus evolution".ZOOL RES 46.1(2025):103-121.

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