| Bat-derived oligopeptide LE6 inhibits the contact-kinin pathway and harbors anti-thromboinflammation and stroke potential |
| Cha, LN; Yang, J; Gao, JA; Lu, X; Chang, XL; Thuku, RC; Liu, Q; Lu, QM; Li, DS; Lai, R; Fang, MQ
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| 2024
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| 发表期刊 | ZOOLOGICAL RESEARCH
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| ISSN | 2095-8137
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| 卷号 | 45期号:5页码:1001-1012 |
| 摘要 | Thrombosis and inflammation are primary contributors to the onset and progression of ischemic stroke. The contactkinin pathway, initiated by plasma kallikrein (PK) and activated factor XII (FXIIa), functions bidirectionally with the coagulation and inflammation cascades, providing a novel target for therapeutic drug development in ischemic stroke. In this study, we identified a bat-derived oligopeptide from Myotis myotis (Borkhausen, 1797), designated LE6 (Leu-Ser-Glu-Glu-Pro-Glu, 702 Da), with considerable potential in stroke therapy due to its effects on the contact kinin pathway. Notably, LE6 demonstrated significant inhibitory effects on PK and FXIIa, with inhibition constants of 43.97 mu mol/L and 6.37 mu mol/L, respectively. In vitro analyses revealed that LE6 prolonged plasma recalcification time and activated partial thromboplastin time. In murine models, LE6 effectively inhibited carrageenan-induced mouse tail thrombosis, FeCl3- 3- induced carotid artery thrombosis, and photochemically induced intracerebral thrombosis. Furthermore, LE6 significantly decreased inflammation and stroke injury in transient middle cerebral artery occlusion models. Notably, the low toxicity, hemolytic activity, and bleeding risk of LE6, along with its synthetic simplicity, underscore its clinical applicability. In conclusion, as an inhibitor of FXIIa and PK, LE6 offers potential therapeutic benefits in stroke treatment by mitigating inflammation and preventing thrombus formation. |
| 收录类别 | SCI
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| 语种 | 英语
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/14529
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| 专题 | 科研部门_天然药物功能蛋白质学科组（赖仞）
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推荐引用方式
GB/T 7714 |
Cha, LN,Yang, J,Gao, JA,et al. Bat-derived oligopeptide LE6 inhibits the contact-kinin pathway and harbors anti-thromboinflammation and stroke potential[J]. ZOOLOGICAL RESEARCH,2024,45(5):1001-1012.
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| APA |
Cha, LN.,Yang, J.,Gao, JA.,Lu, X.,Chang, XL.,...&Fang, MQ.(2024).Bat-derived oligopeptide LE6 inhibits the contact-kinin pathway and harbors anti-thromboinflammation and stroke potential.ZOOLOGICAL RESEARCH,45(5),1001-1012.
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| MLA |
Cha, LN,et al."Bat-derived oligopeptide LE6 inhibits the contact-kinin pathway and harbors anti-thromboinflammation and stroke potential".ZOOLOGICAL RESEARCH 45.5(2024):1001-1012.
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