HSV-1 ICP22 condensates impair host transcription by depleting promoter RNAPII Ser-2P occupation

	HSV-1 ICP22 condensates impair host transcription by depleting promoter RNAPII Ser-2P occupation
	Qi, HS; Yin, MQ; Ren, XL; Chen, GJ; Li, A; Li, YX; Cao, X; Zhou, JM
	2025
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	16
摘要	Herpes simplex virus type I (HSV-1) infection-induced host transcript ion shutdown is one of the most critical hallmarks of viral lytic infection. However, how HSV-1 and which viral factors accomplish this dramatic effect is not well understood. In this study, we show that ICP22-defined condensates shutdown host global transcription but facilitate viral transcription. This is independent of its effects on viral infection-triggered changes in splicing, readthrough, and read-in events. ICP22 condensates depleted the serine-2 phosphorylated RNA polymerase II (RNAPII Ser-2P) occupancy from the host transcription start site (TSS), resulting in decreased host transcripts output. At the same time, it ensures proper RNAPII Ser-2P distribution on the viral genome to promote viral transcription. This effect is dependent solely on the condensate-forming activity, as condensate-disrupting point mutations abolish it. In addition, ectopic expressed ICP22 alone could decrease host transcription activity and increase histone H3K27me3 modification level. Thus, ICP22 condensates shut down host transcription by reducing RNAPII binding to host TSS to impair the host transcription.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14577
专题	科研部门_基因调控与表达遗传（周巨民）
推荐引用方式 GB/T 7714	Qi, HS,Yin, MQ,Ren, XL,et al. HSV-1 ICP22 condensates impair host transcription by depleting promoter RNAPII Ser-2P occupation[J]. FRONTIERS IN MICROBIOLOGY,2025,16.
APA	Qi, HS.,Yin, MQ.,Ren, XL.,Chen, GJ.,Li, A.,...&Zhou, JM.(2025).HSV-1 ICP22 condensates impair host transcription by depleting promoter RNAPII Ser-2P occupation.FRONTIERS IN MICROBIOLOGY,16.
MLA	Qi, HS,et al."HSV-1 ICP22 condensates impair host transcription by depleting promoter RNAPII Ser-2P occupation".FRONTIERS IN MICROBIOLOGY 16(2025).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
ZJM2025040702.pdf（2877KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文