MicroRNA-mediated network redundancy is constrained by purifying selection and contributes to expression robustness in Drosophila melanogaster

	MicroRNA-mediated network redundancy is constrained by purifying selection and contributes to expression robustness in Drosophila melanogaster
	Dai, AM; Lan, WQ; Lyu, Y; Zhou, XY; Mi, X; Tang, T; Liufu, ZQ
	2024
发表期刊	COMMUN BIOL
卷号	7 期号:1
摘要	MicroRNAs (miRNAs) are post-transcriptional, non-coding regulatory RNAs that function coordinately with transcription factors (TFs) in gene regulatory networks. TFs and their targets are often co-regulated by miRNAs, forming composite feedforward circuits (cFFCs) with varying degrees of redundancy, primarily mediated by miRNAs. However, the maintenance of miRNA-mediated regulatory redundancy and its impact on gene expression evolution remain elusive. By integrating ChIP-seq data from ENCODE and miRNA targeting from TargetScanFly, we quantified miRNA-mediated cFFC redundancy in Drosophila melanogaster embryos and larvae, revealing more than three quarters of miRNA targets are involved in redundant cFFCs. Higher cFFC redundancy, where more miRNAs target the same gene within a cFFC, is correlated with stronger purifying selection, reduced expression divergence between species, and increased expression stability under heat shock stress. Redundant cFFCs primarily regulate older or broadly expressed young genes. These findings highlight the role of miRNA-mediated cFFC redundancy in enhancing gene expression robustness through natural selection. Network analysis reveals miRNAs mediate high redundancy of composite feedforward circuits (cFFCs) in Drosophila, correlating with strong purifying selection, reduced interspecies expression divergence, and increased stability under stress.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14626
专题	科研部门_适应性进化与进化医学（吕雪梅）
推荐引用方式 GB/T 7714	Dai, AM,Lan, WQ,Lyu, Y,et al. MicroRNA-mediated network redundancy is constrained by purifying selection and contributes to expression robustness in Drosophila melanogaster[J]. COMMUN BIOL,2024,7(1).
APA	Dai, AM.,Lan, WQ.,Lyu, Y.,Zhou, XY.,Mi, X.,...&Liufu, ZQ.(2024).MicroRNA-mediated network redundancy is constrained by purifying selection and contributes to expression robustness in Drosophila melanogaster.COMMUN BIOL,7(1).
MLA	Dai, AM,et al."MicroRNA-mediated network redundancy is constrained by purifying selection and contributes to expression robustness in Drosophila melanogaster".COMMUN BIOL 7.1(2024).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
LXM2025042101.pdf（1598KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文