Time-dependent changes in genome-wide gene expression and post-transcriptional regulation across the post-death process in silkworm

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	Time-dependent changes in genome-wide gene expression and post-transcriptional regulation across the post-death process in silkworm
	Yang, LY; Tang, DR; Luo, SQ; Li, WW; Jiang, YH; Lin, LB; Zhang, QL
	2024
发表期刊	DNA RES
ISSN	1340-2838
卷号	31 期号:6
摘要	Despite death marking the end of life, several gene expression and miRNA-mediated post-transcriptional regulation events may persist or be initiated. The silkworm (Bombyx mori) is a valuable model for exploring life processes, including death. In this study, we combined transcriptomics and miRNAomics analyses of young, old, and post-mortem silkworms across the entire process after death to unravel the dynamics of gene expression and miRNA-mediated post-transcriptional regulation. In total, 171 genes exhibited sustained differential expression in post-mortem silkworms compared to the pre-death state, which are primarily involved in nerve signalling, transport, and immune response. Post-mortem time-specific genes were associated with cell cycle regulation, thermogenesis, immunity, and zinc ion homeostasis. We found that the down-regulated expression of 36 genes related to transcription, epigenetic modification, and homeostasis resulted in a significant shift in global gene expression patterns at 2 h post-death. We also identified 5 mRNA-miRNA pairs (i.e. bmo-miR-2795-mhca, 2784-achi, 2762-oa1, 277-5p-creb, and 1000-tcb1) associated with stress hormone regulation, transcription activity, and signal transduction. The roles of these pairs were validated through in vivo experiments using miRNA mimics in silkworms. The findings provide valuable insights into the intricate mechanisms underlying the transcriptional and miRNA-mediated post-transcriptional regulation events in animals after death.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14635
专题	管理、支撑部门_动物博物馆
推荐引用方式 GB/T 7714	Yang, LY,Tang, DR,Luo, SQ,et al. Time-dependent changes in genome-wide gene expression and post-transcriptional regulation across the post-death process in silkworm[J]. DNA RES,2024,31(6).
APA	Yang, LY.,Tang, DR.,Luo, SQ.,Li, WW.,Jiang, YH.,...&Zhang, QL.(2024).Time-dependent changes in genome-wide gene expression and post-transcriptional regulation across the post-death process in silkworm.DNA RES,31(6).
MLA	Yang, LY,et al."Time-dependent changes in genome-wide gene expression and post-transcriptional regulation across the post-death process in silkworm".DNA RES 31.6(2024).

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BWG2025041012.pdf（864KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文