Recombinase-aid amplification combined with lateral flow detection assay for sex identification of the great white pelican (Pelecanus onocrotalus)

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	Recombinase-aid amplification combined with lateral flow detection assay for sex identification of the great white pelican (Pelecanus onocrotalus)
	Zeng, FW; Chen, XJ; Zhong, WH; Chen, TZP; Sa, JQ; Wang, GQ; Zhang, SQ; Peng, SM
	2024
发表期刊	SCI REP-UK
ISSN	2045-2322
卷号	14 期号:1
摘要	Sex identification in avian species is essential for biodiversity conservation and ecological studies. However, the sex of nearly half of the birds could not be identified based on their external appearance. It is difficult to visually identify sex to monitor the ecology and conservation of wild populations. In this study, we designed primer pairs for large white pelican using recombinase-based isothermal amplification combined with a lateral flow dipstick (RAA-LFD) assay for chromo-helicase-DNA binding protein (CHD) genes mapped to W chromosomes and an ultra-conserved element (UCE) located on chromosome 6, respectively. Our result showed that the raaW4-RAA-LFD can detect up to 0.1 ng of genomic DNA (gDNA) templates of female pelicans in 30 min at 39 degree celsius and accurately distinguish female from male without any cross reactivity. RaaUCE2-RAA-LFD can amplify both male and female pelicans with a detection limit of 25 pg. To further evaluate the assay, 15 white pelicans of unknown sex were tested using the RAA-LFD assay and conventional polymerase chain reaction (PCR). The results of the raaW4-RAA-LFD assay were consistent with those of the conventional PCR. The developed RAA-LFD assay is equipped with field-deployable instruments and offers a field platform for rapid and reliable sex identification in pelicans.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14711
专题	昆明动物研究所
推荐引用方式 GB/T 7714	Zeng, FW,Chen, XJ,Zhong, WH,et al. Recombinase-aid amplification combined with lateral flow detection assay for sex identification of the great white pelican (Pelecanus onocrotalus)[J]. SCI REP-UK,2024,14(1).
APA	Zeng, FW.,Chen, XJ.,Zhong, WH.,Chen, TZP.,Sa, JQ.,...&Peng, SM.(2024).Recombinase-aid amplification combined with lateral flow detection assay for sex identification of the great white pelican (Pelecanus onocrotalus).SCI REP-UK,14(1).
MLA	Zeng, FW,et al."Recombinase-aid amplification combined with lateral flow detection assay for sex identification of the great white pelican (Pelecanus onocrotalus)".SCI REP-UK 14.1(2024).

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QT2025041029.pdf（1668KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文