Using Recombinase-Aid Amplification Combined with Pyrococcus furiosus Argonaute for Rapid Sex Identification in Flamingo (Phoenicopteridae)

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	Using Recombinase-Aid Amplification Combined with Pyrococcus furiosus Argonaute for Rapid Sex Identification in Flamingo (Phoenicopteridae)
	Tan, SL; Zeng, FW; Zhong, WH; Chen, TZP; Chen, XJ; Li, L; Wei, HX; Zhang, SQ
	2025
发表期刊	ANIMALS-BASEL
ISSN	2076-2615
卷号	15 期号:1
摘要	Flamingos (Phoenicopteridae) are among the oldest birds worldwide and are loved by people for their bright red feathers. In addition, flamingos are sexually monomorphic birds, and distinguishing between males and females is difficult. The polymerase chain reaction (PCR) is widely used for sex identification. However, the PCR method requires a precise thermal cycler in the laboratory and is time-consuming. Therefore, developing a rapid, sensitive, and accurate method to identify the sex of flamingos is crucial. In this study, we established a sex identification system using a recombinase-aided amplification-Pyrococcus furiosus Argonaute (RAA-PfAgo) technique for greater flamingo (Phoenicopterus roseus). The greater flamingo-RAA-PfAgo system can identify unknown-sex greater flamingos in less than 1 h and can be visualized using a fluorescent detector or blue light. The results showed that optimal RAA-PfAgo conditions could detect 0.6 ng of genomic DNA and effectively differentiate between males and females. Random sample evaluations revealed that the system had a 100% coincidence rate compared with conventional PCR. In conclusion, this study provides a sensitive, specific, and accurate reference method for greater flamingo sexing.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14715
专题	昆明动物研究所
推荐引用方式 GB/T 7714	Tan, SL,Zeng, FW,Zhong, WH,et al. Using Recombinase-Aid Amplification Combined with Pyrococcus furiosus Argonaute for Rapid Sex Identification in Flamingo (Phoenicopteridae)[J]. ANIMALS-BASEL,2025,15(1).
APA	Tan, SL.,Zeng, FW.,Zhong, WH.,Chen, TZP.,Chen, XJ.,...&Zhang, SQ.(2025).Using Recombinase-Aid Amplification Combined with Pyrococcus furiosus Argonaute for Rapid Sex Identification in Flamingo (Phoenicopteridae).ANIMALS-BASEL,15(1).
MLA	Tan, SL,et al."Using Recombinase-Aid Amplification Combined with Pyrococcus furiosus Argonaute for Rapid Sex Identification in Flamingo (Phoenicopteridae)".ANIMALS-BASEL 15.1(2025).

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QT2025041034.pdf（3748KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文