Drosophila CG11700 may not affect male fecundity-lifespan tradeoff as previously reported

KIZ OpenIR

	Drosophila CG11700 may not affect male fecundity-lifespan tradeoff as previously reported
	Zuo, DS; Liu, WW; Zhao, RP; Zhu, KS; Wang, W; Xiang, H
	2025
发表期刊	MOLECULAR BIOLOGY AND EVOLUTION
ISSN	0737-4038
卷号	42 期号:2
摘要	Our recent investigations on the function of Drosophila CG11700 and CG32744 (Ubi-p5E) genes using CRISPR/Cas9 deletion technology could not repeat or confirm the results on CG11700 shown in our previous study, which was based on P-element excision assay (Zhan Z, Ding Y, Zhao R, Zhang Y, Yu H, Zhou Q, Yang S, Xiang H, Wang W. Rapid functional divergence of a newly evolved polyubiquitin gene in Drosophila and its role in the trade-off between male fecundity and lifespan. Mol Biol Evol. 2012:29(5):1407-1416. doi:10.1093/molbev/msr299). Here, by CRISPR/Cas9 editing, we generated mutants of CG32744 with the whole gene body fully deleted from the genome, and truncated mutants of CG11700 with N-terminal 103 aa deleted out of its total 301 aa peptide sequence. We carefully conducted the male fecundity assay and found that offsprings of the CG11700 mutant were not significantly more than the wild type, inconsistent with our previous report (Zhan et al. 2012). Meanwhile, we repeated the lifespan assay and did not find that the lifespan of the CG11700 mutant was significantly shorter than the wild type as reported (2012). The new results suggest that the CG11700 gene may not affect male fecundity-lifespan tradeoff as previously reported (Zhan et al. 2012). The new results are thus worthy of reporting to avoid possible misleading by the previous results to the scientific community.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14727
专题	昆明动物研究所
推荐引用方式 GB/T 7714	Zuo, DS,Liu, WW,Zhao, RP,et al. Drosophila CG11700 may not affect male fecundity-lifespan tradeoff as previously reported[J]. MOLECULAR BIOLOGY AND EVOLUTION,2025,42(2).
APA	Zuo, DS,Liu, WW,Zhao, RP,Zhu, KS,Wang, W,&Xiang, H.(2025).Drosophila CG11700 may not affect male fecundity-lifespan tradeoff as previously reported.MOLECULAR BIOLOGY AND EVOLUTION,42(2).
MLA	Zuo, DS,et al."Drosophila CG11700 may not affect male fecundity-lifespan tradeoff as previously reported".MOLECULAR BIOLOGY AND EVOLUTION 42.2(2025).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
WW2025040704.pdf（603KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文