Convergent Evolution and Host-Limiting Impacts of SARS-CoV-2 Revealed by Cellular Experiments

	Convergent Evolution and Host-Limiting Impacts of SARS-CoV-2 Revealed by Cellular Experiments
	Zhang, T; Tian, RR; Li, FY; Tang, XL; He, WB; Hao, ZP; Zhuo, L; Lu, J; Lu, XM; Zheng, YT
	2025
发表期刊	Mol. Biol. Evol.
ISSN	0737-4038
卷号	42 期号:11
摘要	The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted the virus's remarkable ability to evolve and adapt in diverse hosts. Despite the observation of recurrent mutations and convergent evolution in the viral genome, the mechanisms driving these processes remain poorly understood, particularly in the context of diverse host environments and limited genomic surveillance. We established a rigorously controlled in vitro cellular system within a Biosafety Level 3 Laboratory, ensuring strict adherence to biosafety protocols while passaging the virus in seven cell lines derived from four tissues across five mammalian species. High-throughput sequencing revealed consistent positive selection on the Spike (S) protein, highlighting its adaptability in the absence of adaptive immune responses or therapeutic pressures. Type I interferons (IFN-I) and APOBEC-mediated editing may emerge as key modulators of viral evolution. Notably, IFN-I activation is inversely correlated with the accumulation of S protein mutations (E484D, P812R/L, L1186R). Our findings uncover host-specific selective forces in shaping SARS-CoV-2 evolution and highlight the need for systematic approaches to mitigate viral transmission and emerging variants.
收录类别	SCIE
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14805
专题	科研部门_分子免疫药理学（郑永唐）
推荐引用方式 GB/T 7714	Zhang, T,Tian, RR,Li, FY,et al. Convergent Evolution and Host-Limiting Impacts of SARS-CoV-2 Revealed by Cellular Experiments[J]. Mol. Biol. Evol.,2025,42(11).
APA	Zhang, T.,Tian, RR.,Li, FY.,Tang, XL.,He, WB.,...&Zheng, YT.(2025).Convergent Evolution and Host-Limiting Impacts of SARS-CoV-2 Revealed by Cellular Experiments.Mol. Biol. Evol.,42(11).
MLA	Zhang, T,et al."Convergent Evolution and Host-Limiting Impacts of SARS-CoV-2 Revealed by Cellular Experiments".Mol. Biol. Evol. 42.11(2025).

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