Distinct Innate Immune Programs in Nile Tilapia Head Kidney During Infections with Streptococcus agalactiae, Escherichia coli and Vibrio harveyi

KIZ OpenIR > 基因起源组（王文）

	Distinct Innate Immune Programs in Nile Tilapia Head Kidney During Infections with Streptococcus agalactiae, Escherichia coli and Vibrio harveyi
	Cheng, JN; Luo, YP; Shen, J; Yang, KP; Lyu, Z
	2025
发表期刊	Fishes
卷号	10 期号:12
摘要	Nile tilapia (Oreochromis niloticus) is a globally important aquaculture species. However, intensive farming conditions increase the risk of bacterial diseases. Despite the fact that a considerable number of transcriptomic studies have examined host responses to single bacterial infections, comparative analyses conducted within a unified experimental framework remain scarce, limiting the understanding of pathogen-specific defence mechanisms. In this study, tilapia were experimentally infected with Streptococcus agalactiae, Escherichia coli, or Vibrio harveyi via thoracic injection. Head kidney tissues were collected at 48 h post-infection for RNA sequencing. The identification of differentially expressed genes (DEGs) was conducted utilising the edgeR, and the assessment of functional enrichment was facilitated through the implementation of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. A comparative analysis was conducted between the bacterial infection groups and the control group. The results of this analysis revealed the identification of 2930, 3328, and 4850 DEGs were identified in the S. agalactiae, E. coli, and V. harveyi infection groups, respectively. Integrated transcriptomic analysis, combining KEGG enrichment and expression profiling of key genes, revealed distinct response patterns across pathogens. The S. agalactiae infection predominantly activated innate immune signaling pathways, including Toll-like receptor, NOD-like receptor, cytokine-cytokine receptor interaction, and NF-kappa B pathways. In contrast, E. coli infection induced extensive metabolic reprogramming, notably in purine and pyrimidine metabolism, carbon metabolism, and amino acid biosynthesis. Meanwhile, an infection caused by V. harveyi resulted in mucosal and lysosomal defence responses, as evidenced by an increase in lysosome, phagosome, extracellular matrix-receptor interaction, and cell adhesion molecule pathways. Collectively, this study suggests that the head kidney of Nile tilapia employs pathogen-specific defence strategies rather than a uniform antibacterial response, providing one of the first transcriptomic comparisons of distinct bacterial infections in this species. These findings provide fundamental data and theoretical insights for elucidating immune mechanisms in teleost fish and for developing targeted prevention and control strategies in aquaculture.
收录类别	SCIE
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14991
专题	基因起源组（王文）
推荐引用方式 GB/T 7714	Cheng, JN,Luo, YP,Shen, J,et al. Distinct Innate Immune Programs in Nile Tilapia Head Kidney During Infections with Streptococcus agalactiae, Escherichia coli and Vibrio harveyi[J]. Fishes,2025,10(12).
APA	Cheng, JN,Luo, YP,Shen, J,Yang, KP,&Lyu, Z.(2025).Distinct Innate Immune Programs in Nile Tilapia Head Kidney During Infections with Streptococcus agalactiae, Escherichia coli and Vibrio harveyi.Fishes,10(12).
MLA	Cheng, JN,et al."Distinct Innate Immune Programs in Nile Tilapia Head Kidney During Infections with Streptococcus agalactiae, Escherichia coli and Vibrio harveyi".Fishes 10.12(2025).

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2026070701.pdf（1953KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文