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Chromosome-level genome assembly of stem borer Batocera rufomaculata using PacBio HiFi and Hi-C sequencing
He, JW; Bai, TQ; Wan, WT; Dong, ZW; Wang, YJ; Zhang, HR; Li, XY
2025
发表期刊Sci. Data
卷号12期号:1
摘要Batocera rufomaculata (Cerambycidae: Lamiinae), a prominent representative of longhorned beetles, is a globally significant stem-boring pest, infesting over 50 species of deciduous trees. Despite its substantial ecological and economic impact, the genomic basis underlying its host adaptation remain poorly understood. Here, we present a chromosome-level genome assembly of B. rufomaculata, constructed using a combination of Illumina, PacBio HiFi, and Hi-C sequencing data. The genome spans 338.08 Mb, with a scaffold N50 of 37.00 Mb, and is organized into 10 pseudo-chromosomes, including a chromosome X validated by genome collinearity and sequencing depth analyses. Repetitive elements constitute 27.89% of the genome, totaling 94.29 Mb. Out of 17,887 predicted genes, 12,729 were functionally annotated with at least one supporting evidence. The high-quality genome assembly and annotation were confirmed by multiple metrics, including genome size, reads mapping rate (>99.5%), BUSCO completeness (>97.1%), and collinearity analyses. This comprehensive genomic resource provides a foundation for investigating the ecological adaptation of B. rufomaculata and offers valuable insights into the genetic mechanisms that could inform pest management strategies.
收录类别SCIE
语种英语
文献类型期刊论文
条目标识符http://ir.kiz.ac.cn/handle/152453/14992
专题基因起源组(王文)
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He, JW,Bai, TQ,Wan, WT,et al. Chromosome-level genome assembly of stem borer Batocera rufomaculata using PacBio HiFi and Hi-C sequencing[J]. Sci. Data,2025,12(1).
APA He, JW.,Bai, TQ.,Wan, WT.,Dong, ZW.,Wang, YJ.,...&Li, XY.(2025).Chromosome-level genome assembly of stem borer Batocera rufomaculata using PacBio HiFi and Hi-C sequencing.Sci. Data,12(1).
MLA He, JW,et al."Chromosome-level genome assembly of stem borer Batocera rufomaculata using PacBio HiFi and Hi-C sequencing".Sci. Data 12.1(2025).
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