A rationally designed cocktail of nanobodies elicited by heterologous vaccination confers protection against SFTSV in preclinical models

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	A rationally designed cocktail of nanobodies elicited by heterologous vaccination confers protection against SFTSV in preclinical models
	Wu, XL; Zhu, LJ; Liang, SJ; Chang, Z; Wang, YX; Zou, QC; Xu, ZL; Zhang, DD; Hu, JQ; Geng, SY; Li, ZF; Yu, WK; Hu, ZL; Chen, M; Cao, K; Jia, B; Wei, HX; Cai, RT; Long, JY; Yang, XM; Yang, QJ; Yu, ZJ; Shi, LE; Xu, BH; Zheng, YW; Meng, SR; Cai, JM; Zhou, GF; Zhu, KR; Dou, YB; Lou, ZY; Li, MH; Wu, Y; Wu, ZW
	2025
发表期刊	Sci. Transl. Med.
ISSN	1946-6234
卷号	17 期号:825
摘要	Severe fever with thrombocytopenia syndrome virus (SFTSV), an emerging tick-borne phlebovirus, poses a growing public health threat, with no approved vaccines or targeted therapies. Its genetic diversity and rapid reassortment have hindered the development of broadly effective interventions. Here, we isolated and characterized broadly neutralizing camelid nanobodies generated by a heterologous immunization strategy. These nanobodies exhibited cross-subtype neutralization and conferred protection against disease in humanized and lethal murine models of SFTSV infection. Structural analysis revealed binding to distinct, nonoverlapping epitopes on the viral glycoprotein complex. A rationally designed cocktail leveraging this epitope diversity achieved complete viral inhibition through synergistic mechanisms. In both murine and immunocompetent ferret models, the cocktail enabled efficient viral clearance and full protection against lethal challenge. These results demonstrate the therapeutic potential of nanobody cocktails for SFTSV infection and establish a generalizable framework for nanobody-based countermeasures against genetically variable emerging viruses, including other members of the Bunyavirales order.
收录类别	SCIE
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/15212
专题	其他
推荐引用方式 GB/T 7714	Wu, XL,Zhu, LJ,Liang, SJ,et al. A rationally designed cocktail of nanobodies elicited by heterologous vaccination confers protection against SFTSV in preclinical models[J]. Sci. Transl. Med.,2025,17(825).
APA	Wu, XL.,Zhu, LJ.,Liang, SJ.,Chang, Z.,Wang, YX.,...&Wu, ZW.(2025).A rationally designed cocktail of nanobodies elicited by heterologous vaccination confers protection against SFTSV in preclinical models.Sci. Transl. Med.,17(825).
MLA	Wu, XL,et al."A rationally designed cocktail of nanobodies elicited by heterologous vaccination confers protection against SFTSV in preclinical models".Sci. Transl. Med. 17.825(2025).

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2026071510.pdf（2268KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文