分子进化与基因组多样性（张亚平）知识库

实验采用水平板淀粉凝胶电泳技术,对中国地方猪五大类型9个品种、1个引入品种共201个个体进行了遗传多样性分析。共分析遗传座位30个,其中只有MDH、PEPB、AK、6PGD、ADA、PHI 6个座位具有多态性。中国主要地方猪品种的多态座位百分比P=0.200,平均杂合度H=0.065,平均等位基因数A=1.300。根据基因频率采用PHYLIP3.5c软件包计算Nei氏遗传距离,然后用“NEIGHBOR”程序分别构建NJ和UPGMA系统树。

A total of 201 blood samples including 9 indigenous pig breeds (Sus scrofa domesticus) in China and Yorkshine pigs were collected in order to evaluate the genetic diversity among Chinese pig breeds.Thirty blood protein loci were studied by horizontal starch gel electrophoresis,among which 6 loci (MDH,PEPB,AK,6PGD,ADA,PHI) were found to be polymorphic.The percentage of polymorphic loci (P),the mean individual heterozygosity (H) and the mean number of alleles (A) are 0.200,0.065 and 1.300,respectively.The degree of genetic variability of Chinese indigenous pig breeds as a whole was higher than that of goats,lower than those of cattle,horses and sheep.Using gene frequencies of the 30 loci,the pairwise genetic distance among the 9 indigenous breeds in China were calculated by software PHYLIP3.5 c.The phylogenetic trees were constructed using NJ and UPGMA methods.The results suggest that the geographical distribution do not consist with the genetic relationship among Chinese indigenous pig breeds.The genetic diversity among Chinese indigenous pig breeds is low.