Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development

	Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development
	Zheng P 1,3; Wang H 1,3; Bavister BD 2,3; Ji W*1,3; wji@mail.kiz.ac.cn
	2001
发表期刊	HUMAN REPRODUCTION
ISSN	0268-1161
卷号	16 期号:2 页码:300-305
合作性质	其它
摘要	This study compared success of in-vitro maturation of rhesus monkey oocytes in protein-free versus serum-containing culture systems, assessed by embryo development subsequent to IVF. Four media were tested: (i) modified Connaught Medical Research Laboratories medium (mCMRL-1066); (ii) hamster embryo culture medium-10 (HECM-10); (iii) control: mCMRL-1066 + 20% bovine calf serum (BCS); (iv) HECM-10 + 20% BCS. Immature oocytes from FSH-stimulated rhesus monkeys were allocated among the media containing ovine FSH (5 microg/ml) and LH (10 microg/ml) and cultured for 36-40 h. Metaphase II ova were inseminated and putative zygotes were cultured in mCMRL-1066 + 20% BCS until development arrested. Ova matured in all four media had similar (P> 0.05) potential to initiate (66, 67, 82 and 69% respectively) and complete meiotic maturation (60, 50, 76 and 57% respectively). Inseminated ova in all groups had similar potential to be fertilized (86, 83, 84 and 90% respectively), cleave (71, 83, 76 and 90% respectively) and develop to the blastocyst stage (19, 17, 16 and 30% respectively). These results indicate for the first time that primate oocytes can be successfully matured in protein-free medium, with subsequent blastocyst development, comparable to responses in complex medium with serum. This finding will facilitate studies on mechanisms regulating primate oocyte maturation.
关键词	Blastocysts In-vitro Maturation/primate Oocytes Protein-free Medium
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收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2397
专题	科研部门_哺乳动物胚胎发育（郑萍）生殖与发育生物学
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Chinese Acad Sci, Kunming Inst Zool, Dept Reprod Biol, Kunming 650223, Yunnan, Peoples R China 2.Univ Wisconsin, Dept Anim Hlth & Biomed Sci, Madison, WI 53706 USA 3.Chinese Acad Sci, Kunming Inst Zool, China US Primate Biol Lab, Kunming 650223, Peoples R China
推荐引用方式 GB/T 7714	Zheng P,Wang H,Bavister BD,et al. Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development[J]. HUMAN REPRODUCTION,2001,16(2):300-305.
APA	Zheng P,Wang H,Bavister BD,Ji W*,&wji@mail.kiz.ac.cn.(2001).Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development.HUMAN REPRODUCTION,16(2),300-305.
MLA	Zheng P,et al."Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development".HUMAN REPRODUCTION 16.2(2001):300-305.

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