Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima

	Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima
	Lai R; Liu H; Lee WH; Zhang Y*; zhangy@mail.kiz.ac.cn
	2002
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B
ISSN	1096-4959
卷号	131 期号:1 页码:47-53
合作性质	其它
摘要	A novel trypsin inhibitor was identified and purified from skin secretions of Chinese red-belly toad Bombina maxima. The partial N-terminal 29 amino acid residues of the peptide, named BMTI, were determined by automated Edman degradation. This allowed the cloning of a full-length cDNA encoding BMTI from a cDNA library prepared from the toad skin. The deduced complete amino acid sequence of BMTI indicates that mature BMTI is composed of 60 amino acids. A FASTA search in the databanks revealed that BMTI exhibits 81.7% sequence identity with BSTI, a trypsin/thrombin inhibitor from European toad Bombina bombina skin secretions. Sequence differences between BMTI and BSTI were due to 11 substitutions at positions 2, 9, 25, 27, 36-37, 39, 41-42, 50 and 56. BMTI potently inhibited trypsin with a K(i) value of 0.06 microM, similar to that of BSTI. However, unlike BSTI, which also inhibited thrombin with a K(i) value of 1 microM, no inhibitory effect of BMTI on thrombin was observed under the assay conditions.
关键词	Protease Inhibitor Trypsin Amphibian Skin Bombina Toad Bombina Maxima Trypsin Inhibitor Bmti Cdna Sequence
资助者	This work was supported by grants from ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Plan’ pre-research projects from the Chinese Academy of Sciences and a grant from National Ž . Natural Science foundation 30170195 . ; This work was supported by grants from ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Plan’ pre-research projects from the Chinese Academy of Sciences and a grant from National Ž . Natural Science foundation 30170195 . ; This work was supported by grants from ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Plan’ pre-research projects from the Chinese Academy of Sciences and a grant from National Ž . Natural Science foundation 30170195 . ; This work was supported by grants from ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Plan’ pre-research projects from the Chinese Academy of Sciences and a grant from National Ž . Natural Science foundation 30170195 .
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收录类别	SCI
语种	英语
资助者	This work was supported by grants from ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Plan’ pre-research projects from the Chinese Academy of Sciences and a grant from National Ž . Natural Science foundation 30170195 . ; This work was supported by grants from ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Plan’ pre-research projects from the Chinese Academy of Sciences and a grant from National Ž . Natural Science foundation 30170195 . ; This work was supported by grants from ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Plan’ pre-research projects from the Chinese Academy of Sciences and a grant from National Ž . Natural Science foundation 30170195 . ; This work was supported by grants from ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Plan’ pre-research projects from the Chinese Academy of Sciences and a grant from National Ž . Natural Science foundation 30170195 .
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2471
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_生物毒素与人类疾病（张云）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming 650223, Yunnan, PR China
推荐引用方式 GB/T 7714	Lai R,Liu H,Lee WH,et al. Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B,2002,131(1):47-53.
APA	Lai R,Liu H,Lee WH,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2002).Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B,131(1),47-53.
MLA	Lai R,et al."Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B 131.1(2002):47-53.

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