A new family of antimicrobial peptides from skin secretions of Rana pleuraden

	A new family of antimicrobial peptides from skin secretions of Rana pleuraden
	Wang X 1; Song YZ 2; Li JX 2,3; Liu H 1; Xu XQ 2,3; Lai R1,2; Zhang KY1; rlai@mail.kiz.ac.cn ; keyunzhang@njau.edu.cn
	2007
发表期刊	PEPTIDES
ISSN	0196-9781
卷号	28 期号:10 页码:2069-2074
合作性质	其它
摘要	While conducting experiments to investigate antimicrobial peptides of amphibians living in the Yunnan-Guizhou region of southwest China, a new family of antimicrobial peptides was identified from skin secretions of the Yunnan frog, Rana pleuraden. Members of the new peptide family named pleurain-As are composed of 26 amino acids with a unique N-terminal sequence (SIIT) and a disulfide-bridged heptapeptide sequence (CRLYNTC). By BLAST search, pleurain-As had no significant similarity to any known peptides. Native and synthetic peptides showed antimicrobial activities against tested microorganisms including Gram-negative and Gram-positive bacteria and fungi. Twenty different cDNAs encoding pleurain-As were cloned from the skin cDNA library of R. pleuraden. The precursors of pleurain-As are composed of 69 amino acid residues including predicted signal peptides, acidic propieces, and cationic mature antimicrobial peptides. The preproregion of pleurain-A precursor comprises a hydrophobic signal peptide of 22 residues followed by an 18 residue acidic propiece which terminates by a typical prohormone processing signal Lys-Arg. The preproregions of precursors are very similar to other amphibian antimicrobial peptide precursors but the mature pleurain-As are different from other antimicrobial peptide families. The remarkable similarity of preproregions of precursors that give rise to very different antimicrobial peptides in distantly related frog species suggests that the corresponding genes form a multigene family originating from a common ancestor. Furthermore, pleurain-As could exert antimicrobial capability against Helicobacter pylori. This is the first report of naturally occurring peptides with anti-H. pylori activity from Rana amphibians.
关键词	Amphibian Antimicrobial Peptides Rana Pleuraden Helicobacter Pylori
资助者	This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences.
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收录类别	SCI
语种	英语
资助者	This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2501
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai@mail.kiz.ac.cn; keyunzhang@njau.edu.cn
作者单位	1.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 2.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
推荐引用方式 GB/T 7714	Wang X,Song YZ,Li JX,et al. A new family of antimicrobial peptides from skin secretions of Rana pleuraden[J]. PEPTIDES,2007,28(10):2069-2074.
APA	Wang X.,Song YZ.,Li JX.,Liu H.,Xu XQ.,...&keyunzhang@njau.edu.cn.(2007).A new family of antimicrobial peptides from skin secretions of Rana pleuraden.PEPTIDES,28(10),2069-2074.
MLA	Wang X,et al."A new family of antimicrobial peptides from skin secretions of Rana pleuraden".PEPTIDES 28.10(2007):2069-2074.

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