Isolation and cDNA cloning of cholecystokinin from the skin of Rana nigrovittata

	Isolation and cDNA cloning of cholecystokinin from the skin of Rana nigrovittata
	Liu XH 1; Wang YP 2,3; Cheng LH 1; Song YZ 2,3; Lai R*1,2; rlai72@njau.edu.cn
	2007
发表期刊	PEPTIDES
ISSN	0196-9781
卷号	28 期号:8 页码:1540-1544
合作性质	其它
摘要	Many neuroendocrine peptides that are distributed in amphibian gastrointestinal tract and central nervous system are also found in amphibian skins, and these peptides are classified into skin-gut-brain triangle peptides, such as bombesins, gastrin-releasing peptides. Cholecystokinins (CCKs) are neuroendocrine peptides known for their production in the gastrointestinal tract and central nervous system of mammalians. Several CCKs have been identified from two amphibians, Rana catesbeiana and Xenopus laevis. These amphibian CCKs are found to be express in brain and in the gastrointestinal tract, but not in skin. In the current report, a cholecystokinin (CCK) isoform was identified from skin secretions of the frog, Rana nigrovittata. Its amino acid sequence is RVDGNSDQKAVIGAMLAKDLQTRKAGSST-GRYAVLPNR PVIDPTHRINDRDYMGVTMDF, which is the same with that of CCK from R. catesbeiana. Four different cDNAs (GenBank accession nos. EF608063-6) encoding CCK precursors were cloned from the cDNA library of the skin of R. nigrovittata. The present data demonstrated that amphibian CCK could also be expressed in gastrointestinal tract, central nervous system and skin as other amphibian skin-gut-brain triangle peptides.
关键词	Amphibian Rana Nigrovittata Cholecystokinin Skin-intestine-brain Triangle Peptide
资助者	This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences.
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收录类别	SCI
语种	英语
资助者	This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Acad- emy of Sciences.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2505
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai72@njau.edu.cn
作者单位	1.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 2.Biotoxin Department of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
推荐引用方式 GB/T 7714	Liu XH,Wang YP,Cheng LH,et al. Isolation and cDNA cloning of cholecystokinin from the skin of Rana nigrovittata[J]. PEPTIDES,2007,28(8):1540-1544.
APA	Liu XH,Wang YP,Cheng LH,Song YZ,Lai R*,&rlai72@njau.edu.cn.(2007).Isolation and cDNA cloning of cholecystokinin from the skin of Rana nigrovittata.PEPTIDES,28(8),1540-1544.
MLA	Liu XH,et al."Isolation and cDNA cloning of cholecystokinin from the skin of Rana nigrovittata".PEPTIDES 28.8(2007):1540-1544.

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