Multiple bombesin-like peptides with opposite functions from skin of Odorrana grahami

	Multiple bombesin-like peptides with opposite functions from skin of Odorrana grahami
	Li JX 1,2; Yu HN 3; Xu XQ 1,2; Wang X 4; Liu DJ 5; Lai R*1,4; rlai@mail.kiz.ac.cn
	2007
发表期刊	GENOMICS
ISSN	0888-7543
卷号	89 期号:3 页码:413-418
合作性质	其它
摘要	Bombesin-like peptides (BLPs) are a family of neuroendocrinic peptides that mediate a variety of biological activities. Three mature BLPs from the skin secretions of the frog Odorrana grahami were purified. Several bombesin-like peptide cDNA sequences encoding precursors of BLPs were identified from the skin cDNA library of O. grahami. This is the maximal diversity of BLPs ever found in animals. Five mature BLPs (B1-B5) based on the amino acid sequences derived from the cDNA cloning were synthesized. In the in vitro myotropic contraction experiment, all synthesized BLPs displayed a stimulating effect toward rat stomach strips, except B4 and B5 which showed the opposite effect, suggesting that certain BLPs may act as antagonists of bombesin receptors while most other BLPs act as agonists. This finding will facilitate the finding of novel bombesin receptors and novel ligands of bombesin receptors. The diversity of amphibian BLPs and their precursors were also analyzed and results suggest that amphibian BLPs and corresponding precursors of various sizes and processing patterns can be used as markers of taxonomic and molecular phylogenetics. The remarkable similarity of preproregions gives rise to very different BLPs and 3'-terminal regions in distantly related frog species, suggesting that the corresponding genes form a multigene family originating from a common ancestor. The diversification of BLP loci could thus be part of an evolutionary strategy developed by amphibian species as a result of shifts to novel ecological niches when environmental factors change rapidly.
关键词	Bombesin Amphibian Diversity Odorrana Grahami Molecular Marker
资助者	This work was supported by the Chinese National Natural Science Foundation (30570360, 30676456), Yunnan Natural Science Foundation (2005C0054M), and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30676456), Yunnan Natural Science Foundation (2005C0054M), and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30676456), Yunnan Natural Science Foundation (2005C0054M), and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30676456), Yunnan Natural Science Foundation (2005C0054M), and Jiangsu Natural Science Foundation (BK2005422).
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收录类别	SCI
语种	英语
资助者	This work was supported by the Chinese National Natural Science Foundation (30570360, 30676456), Yunnan Natural Science Foundation (2005C0054M), and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30676456), Yunnan Natural Science Foundation (2005C0054M), and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30676456), Yunnan Natural Science Foundation (2005C0054M), and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30676456), Yunnan Natural Science Foundation (2005C0054M), and Jiangsu Natural Science Foundation (BK2005422).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2517
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai@mail.kiz.ac.cn
作者单位	1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China 3.College of Life Sciences, School of Heibei Normal University, Shijiazhuang, Hebei 050016, China 4.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, 5.School of Animal Science and Technology, South-west University, Chongqing 400715, China
推荐引用方式 GB/T 7714	Li JX,Yu HN,Xu XQ,et al. Multiple bombesin-like peptides with opposite functions from skin of Odorrana grahami[J]. GENOMICS,2007,89(3):413-418.
APA	Li JX.,Yu HN.,Xu XQ.,Wang X.,Liu DJ.,...&rlai@mail.kiz.ac.cn.(2007).Multiple bombesin-like peptides with opposite functions from skin of Odorrana grahami.GENOMICS,89(3),413-418.
MLA	Li JX,et al."Multiple bombesin-like peptides with opposite functions from skin of Odorrana grahami".GENOMICS 89.3(2007):413-418.

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