A novel bradykinin-like peptide from skin secretions of the frog, Rana nigrovittata

	A novel bradykinin-like peptide from skin secretions of the frog, Rana nigrovittata
	Liu XH 1; You DW 2,3; Chen LH 1; Wang X 1; Zhang KY 1; Lai R*1,2; rlai72@njau.edu.cn
	2008
发表期刊	JOURNAL OF PEPTIDE SCIENCE
ISSN	1075-2617
卷号	14 期号:5 页码:626-630
合作性质	其它
摘要	A bradykinin-like peptide has been isolated from the skin secretions of the frog Rana nigrovittata. This peptide was named ranakinin-N. Its primary structure, RAEAVPPGFTPFR, was determined by Edman degradation and mass spectrometry. It is structurally related to bradykinin-like peptides identified from skin secretions of other amphibians. Ranakinin-N is composed of 13 amino acid residues and is related to the bradykinin identified from the skin secretions of Odorrana schmackeri, which is composed of 9 amino acid residues. Ranakinin-N was found to exert concentration-dependent contractile effects on isolated guinea pig ileum. cDNA sequence encoding the precursor of ranakinin-N was isolated from a skin cDNA library of R. nigrovittata. The amino acid sequences deduced from the cDNA sequences match well with the results from Edman degradation. Analysis of different amphibian bradykinin cDNA structures revealed that the deficiency of a 15-nucleotide fragment (agaatgatcagacgc in the cDNA encoding bradykinin from O. schmackeri) in the peptide-coding region resulted in the absence of a dibasic site for trypsin-like proteinases and an unusual -AEVA- insertion in the N-terminal part of ranakinin-N. The -AEAV- insertion resulted in neutral net charge at the N-terminus of ranakinin-N. Ranakinin-N is the first reported bradykinin-like peptide with a neutral net charge at the N-terminus.
关键词	Amphibian Ranakinin-n Bradykinin-like Peptide Rana Nigrovittata Skin
资助者	This work was supported by Jiangsu Natural Science Foundation (BK2005422), the Ministry of Science and Technology of the People’s Republic of China (2006BAD06B07) and the Chinese Academy of Sciences (KSCX2-YW-R-20 and KSCX2-YW-G-024). ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), the Ministry of Science and Technology of the People’s Republic of China (2006BAD06B07) and the Chinese Academy of Sciences (KSCX2-YW-R-20 and KSCX2-YW-G-024). ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), the Ministry of Science and Technology of the People’s Republic of China (2006BAD06B07) and the Chinese Academy of Sciences (KSCX2-YW-R-20 and KSCX2-YW-G-024). ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), the Ministry of Science and Technology of the People’s Republic of China (2006BAD06B07) and the Chinese Academy of Sciences (KSCX2-YW-R-20 and KSCX2-YW-G-024).
URL	查看原文
收录类别	SCI
语种	英语
资助者	This work was supported by Jiangsu Natural Science Foundation (BK2005422), the Ministry of Science and Technology of the People’s Republic of China (2006BAD06B07) and the Chinese Academy of Sciences (KSCX2-YW-R-20 and KSCX2-YW-G-024). ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), the Ministry of Science and Technology of the People’s Republic of China (2006BAD06B07) and the Chinese Academy of Sciences (KSCX2-YW-R-20 and KSCX2-YW-G-024). ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), the Ministry of Science and Technology of the People’s Republic of China (2006BAD06B07) and the Chinese Academy of Sciences (KSCX2-YW-R-20 and KSCX2-YW-G-024). ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), the Ministry of Science and Technology of the People’s Republic of China (2006BAD06B07) and the Chinese Academy of Sciences (KSCX2-YW-R-20 and KSCX2-YW-G-024).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2537
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai72@njau.edu.cn
作者单位	1.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China 2.Biotoxin department of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 3.Graduate School of the Chinese Academy of Sciences, Beijing100009, China
推荐引用方式 GB/T 7714	Liu XH,You DW,Chen LH,et al. A novel bradykinin-like peptide from skin secretions of the frog, Rana nigrovittata[J]. JOURNAL OF PEPTIDE SCIENCE,2008,14(5):626-630.
APA	Liu XH.,You DW.,Chen LH.,Wang X.,Zhang KY.,...&rlai72@njau.edu.cn.(2008).A novel bradykinin-like peptide from skin secretions of the frog, Rana nigrovittata.JOURNAL OF PEPTIDE SCIENCE,14(5),626-630.
MLA	Liu XH,et al."A novel bradykinin-like peptide from skin secretions of the frog, Rana nigrovittata".JOURNAL OF PEPTIDE SCIENCE 14.5(2008):626-630.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
200814626.pdf（225KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文