An anticoagulant serine protease from the wasp venom of Vespa magnifica

	An anticoagulant serine protease from the wasp venom of Vespa magnifica
	Han JY 1,3; You DW 1,4; Xu XQ 1,4; Han W 3; Lu Y 2; Lai R1,2; Meng QX1; rlai@mail.kiz.ac.cn ; qxmene@sina.com
	2008
发表期刊	TOXICON
ISSN	0041-0101
卷号	51 期号:5 页码:914-922
合作性质	其它
摘要	Wasp is an important venomous animal that can induce human fatalities. Coagulopathy is a clinical symptom after massive wasp stings, but the reason leading to the envenomation manifestation is still not known. In this paper, a toxin protein is purified and characterized by Sephadex G-75 gel filtration, CM-Sephadex C-25 cationic exchange and fast protein liquid chromatography (FPLC) from the venom of the wasp, Vespa magnifica (Smith). This protein, named magnvesin. contains serine protease-like activity and inhibits blood coagulation. The cDNA encoding magnvesin is cloned from the venom sac cDNA library of the wasp. The deduced protein from the cDNA is composed of 305 amino acid residues. Magnvesin shares 52% identity with allergen serine protease from the wasp Polistes dominulus. Magnvesin exerted its anti-coagulant function by hydrolyzing coagulant factors TF, VII, VIII, IX and X.
关键词	Wasp Venom Vespa Magnifica Serine Protease Blood Coagulation Anticoagulant
资助者	This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456) and Chinese Academy of Sciences (KSCXZ-YW-R- 088). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456) and Chinese Academy of Sciences (KSCXZ-YW-R- 088). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456) and Chinese Academy of Sciences (KSCXZ-YW-R- 088). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456) and Chinese Academy of Sciences (KSCXZ-YW-R- 088).
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收录类别	SCI
语种	英语
资助者	This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456) and Chinese Academy of Sciences (KSCXZ-YW-R- 088). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456) and Chinese Academy of Sciences (KSCXZ-YW-R- 088). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456) and Chinese Academy of Sciences (KSCXZ-YW-R- 088). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456) and Chinese Academy of Sciences (KSCXZ-YW-R- 088).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2541
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai@mail.kiz.ac.cn; qxmene@sina.com
作者单位	1.Biotoxin Units of Key Laboratories of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China 3.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China 4.Graduate School of the Chinese Academy of Sciences, Beijing100009, China
推荐引用方式 GB/T 7714	Han JY,You DW,Xu XQ,et al. An anticoagulant serine protease from the wasp venom of Vespa magnifica[J]. TOXICON,2008,51(5):914-922.
APA	Han JY.,You DW.,Xu XQ.,Han W.,Lu Y.,...&qxmene@sina.com.(2008).An anticoagulant serine protease from the wasp venom of Vespa magnifica.TOXICON,51(5),914-922.
MLA	Han JY,et al."An anticoagulant serine protease from the wasp venom of Vespa magnifica".TOXICON 51.5(2008):914-922.

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