Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora

	Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora
	Lu XY 1,3; Ma YF 1,2; Wu J 1,2; Lai R*1; rlai@mail.kiz.ac.cn
	2008
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN	1096-4959
卷号	149 期号:4 页码:608-612
摘要	Two serine protease inhibitors (named BMSI 1 and BMSI 2, respectively) were identified from the skin secretions of the toad, Bombina microdeladigitora. The cDNAs encoding BMSIs were cloned from a cDNA library prepared from the toad skin. The deduced complete amino acid sequences of BMSIs indicate that mature BMSI1 and BMSI2 are composed of 60 amino acids including 10 half-cystines to form 5 disulfide bridges. A FASTA search in the databanks revealed that BMSIs exhibit sequence similarity with other serine protease inhibitors from amphibians of the genus Bombina. BMSI1 potently inhibited trypsin and thrombin with a K(i) value of 0.02 mu M and 0.15 mu M, respectively. Sequence analysis revealed that all serine protease inhibitors from five amphibians of the genus Bombina share highly conserved primary structures.
关键词	Serine Protease Inhibitor Amphibian Bombina Microdeladigitora Toad
资助者	This work was supported by 2006BAD06B07 from the Ministry of Science and Technology of the People's Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Academy of Sciences. ; This work was supported by 2006BAD06B07 from the Ministry of Science and Technology of the People's Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Academy of Sciences. ; This work was supported by 2006BAD06B07 from the Ministry of Science and Technology of the People's Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Academy of Sciences. ; This work was supported by 2006BAD06B07 from the Ministry of Science and Technology of the People's Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Academy of Sciences.
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收录类别	SCI
语种	英语
资助者	This work was supported by 2006BAD06B07 from the Ministry of Science and Technology of the People's Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Academy of Sciences. ; This work was supported by 2006BAD06B07 from the Ministry of Science and Technology of the People's Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Academy of Sciences. ; This work was supported by 2006BAD06B07 from the Ministry of Science and Technology of the People's Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Academy of Sciences. ; This work was supported by 2006BAD06B07 from the Ministry of Science and Technology of the People's Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Academy of Sciences.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2545
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai@mail.kiz.ac.cn
作者单位	1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.Graduate School of the Chinese Academy of Sciences, Beijing100009, China 3.Department of Life Science & Technology, Changshu Institute of Technology, Changshu 215500, China
推荐引用方式 GB/T 7714	Lu XY,Ma YF,Wu J,et al. Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2008,149(4):608-612.
APA	Lu XY,Ma YF,Wu J,Lai R*,&rlai@mail.kiz.ac.cn.(2008).Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,149(4),608-612.
MLA	Lu XY,et al."Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 149.4(2008):608-612.

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