A serine proteinase inhibitor from frog eggs with bacteriostatic activity

	A serine proteinase inhibitor from frog eggs with bacteriostatic activity
	Han YP 1,2; Yu HN 1; Yang XB 3; Rees HH 4; Liu JZ1; Lai R3; jzliu21@heinfo.net ; rlai@mail.kiz.ac.cn
	2008
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN	1096-4959
卷号	149 期号:1 页码:58-62
合作性质	其它
摘要	By Sephadex G-50 gel filtration, Resource Q anionic exchange and C4 reversed phase liquid high performance liquid chromatography, a proteinase inhibitor protein (Ranaserpin) was identified and purified from the eggs of the odour frog, Rana grahami. The protein displayed a single band adjacent to the molecular weight marker of 14.4 kDa analyzed by SDS-PAGE. The inhibitor protein homogeneity and its molecular weight were confirmed again by MALDI-TOF mass spectrometry analysis. The MALDI-TOF mass spectrum analysis gave this inhibitor protein an m/z of 14422.26 that was matched well with the result from SDS-PAGE. This protein is a serine proteinase inhibitor targeting multiple proteinases including trypsin, elastase, and subtilisin. Ranaserpin inhibited the proteolytic activities of trypsin, elastase, and subtilisin. It has an inhibitory constant (K-i) of 6.2 x 10(-8) M, 2.7 x 10(-7) M and 2.2 x 10(-8) M for trypsin, elastase, and subtilisin, respectively. This serine proteinase inhibitor exhibited bacteriostatic effect on Gram-positive bacteria Bacillus subtilis (ATCC 6633). It was suggested that ranaserpin might act as a defensive role in resistance to invasion of pests or pathogens. This is the first report of serine proteinase inhibitor and its direct defensive role from amphibian eggs.
关键词	Serine Proteinase Inhibitor Amphibian Egg Defensive Rana Grahami
URL	查看原文
收录类别	SCI
语种	中文
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2547
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	jzliu21@heinfo.net; rlai@mail.kiz.ac.cn
作者单位	1.College of Life Sciences School of Heibei Normal University, Shijiazhuang, Hebei, 050016 China 2.Department of Life Science & Technology, Changshu Institute of Technology, Changshu 215500, China 3.Biotoxins Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 4.School of Biological Sciences of University of Liverpool, Liverpool L69 7ZB, United Kingdom
推荐引用方式 GB/T 7714	Han YP,Yu HN,Yang XB,et al. A serine proteinase inhibitor from frog eggs with bacteriostatic activity[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2008,149(1):58-62.
APA	Han YP.,Yu HN.,Yang XB.,Rees HH.,Liu JZ*.,...&rlai@mail.kiz.ac.cn.(2008).A serine proteinase inhibitor from frog eggs with bacteriostatic activity.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,149(1),58-62.
MLA	Han YP,et al."A serine proteinase inhibitor from frog eggs with bacteriostatic activity".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 149.1(2008):58-62.

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