Toward an understanding of the molecular mechanism for successful blood feeding by coupling proteomics analysis with pharmacological testing of horsefly salivary glands

	Toward an understanding of the molecular mechanism for successful blood feeding by coupling proteomics analysis with pharmacological testing of horsefly salivary glands
	Xu XQ 1,3; Yang HL 1; Ma DY 1,3; Wu J 1,3; Wang YP 1,3; Song YZ 1,3; Wang X 2; Lu Y 2; Yang JX 1; Lai R[*]1,2; rlai@mail.kiz.ac.cn
	2008
发表期刊	MOLECULAR & CELLULAR PROTEOMICS
ISSN	1535-9476
卷号	7 期号:3 页码:582-590
摘要	Horseflies are economically important blood-feeding arthropods and also a nuisance for humans and vectors for filariasis. They rely heavily on the pharmacological properties of their saliva to get a blood meal and suppress immune reactions of hosts. Little information is available on antihemostatic substances in horsefly salivary glands; especially no horsefly immune suppressants have been reported. By proteomics or peptidomics and coupling transcriptome analysis with pharmacological testing, several families of proteins or peptides, which act mainly on the hemostatic system or immune system of the host, were identified and characterized from 30,000 pairs salivary glands of the horsefly Tabanus yao (Diptera, Tabanidae). They are: (i) a novel family of inhibitors of platelet aggregation including two members, which possibly inhibit platelet aggregation by a novel mechanism and act on platelet membrane, (ii) a novel family of immunosuppressant peptides including 12 members, which can inhibit interferon-gamma production and increase interleukin-10 secretion, (iii) a serine protease inhibitor with 56 amino acid residues containing anticoagulant activity, (iv) a serine protease with anticoagulant activity, (v) a protease with fibrinogenolytic activity, (vi) three families of antimicrobial peptides including six members, (vii) a hyaluronidase, (viii) a vasodilator peptide, which is an isoform of vasotab identified from Hybomitra bimaculata, and interestingly (ix) two metallothioneins, which are the first metallothioneins reported from invertebrate salivary glands. The current work will facilitate the understanding of the molecular mechanisms of the ectoparasite-host relationship and help in identifying novel vaccine targets and novel leading pharmacological compounds.
资助者	This work was supported by Grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China and by Grants KSCX2-YW-R-20 and KSCX2-YW-G-024 from the Chinese Academy of Sciences. ; This work was supported by Grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China and by Grants KSCX2-YW-R-20 and KSCX2-YW-G-024 from the Chinese Academy of Sciences. ; This work was supported by Grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China and by Grants KSCX2-YW-R-20 and KSCX2-YW-G-024 from the Chinese Academy of Sciences. ; This work was supported by Grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China and by Grants KSCX2-YW-R-20 and KSCX2-YW-G-024 from the Chinese Academy of Sciences.
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收录类别	SCI
语种	英语
资助者	This work was supported by Grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China and by Grants KSCX2-YW-R-20 and KSCX2-YW-G-024 from the Chinese Academy of Sciences. ; This work was supported by Grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China and by Grants KSCX2-YW-R-20 and KSCX2-YW-G-024 from the Chinese Academy of Sciences. ; This work was supported by Grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China and by Grants KSCX2-YW-R-20 and KSCX2-YW-G-024 from the Chinese Academy of Sciences. ; This work was supported by Grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China and by Grants KSCX2-YW-R-20 and KSCX2-YW-G-024 from the Chinese Academy of Sciences.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2549
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室科研部门_系统进化与生物地理学（杨君兴）
通讯作者	rlai@mail.kiz.ac.cn
作者单位	1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.Key Labo- ratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
推荐引用方式 GB/T 7714	Xu XQ,Yang HL,Ma DY,et al. Toward an understanding of the molecular mechanism for successful blood feeding by coupling proteomics analysis with pharmacological testing of horsefly salivary glands[J]. MOLECULAR & CELLULAR PROTEOMICS,2008,7(3):582-590.
APA	Xu XQ.,Yang HL.,Ma DY.,Wu J.,Wang YP.,...&rlai@mail.kiz.ac.cn.(2008).Toward an understanding of the molecular mechanism for successful blood feeding by coupling proteomics analysis with pharmacological testing of horsefly salivary glands.MOLECULAR & CELLULAR PROTEOMICS,7(3),582-590.
MLA	Xu XQ,et al."Toward an understanding of the molecular mechanism for successful blood feeding by coupling proteomics analysis with pharmacological testing of horsefly salivary glands".MOLECULAR & CELLULAR PROTEOMICS 7.3(2008):582-590.

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