Purification, characterization and cloning of two novel tigerinin-like peptides from skin secretions of Fejervarya cancrivora

	Purification, characterization and cloning of two novel tigerinin-like peptides from skin secretions of Fejervarya cancrivora
	Song YZ 1,4; Lu Y 2; Wang LJ 3; Yang HL 1,4; Zhang KY 2; Lai R*1,2; rlai@mail.kiz.ac.cn
	2009
发表期刊	PEPTIDES
ISSN	0196-9781
卷号	30 期号:7 页码:1228-1232
合作性质	其它
摘要	While investigating the innate defense of brackish water-living amphibian and its comparison with freshwater-living amphibians, two novel 12-residue antimicrobial peptides were purified from the skin secretions of the crab-eating frog, Fejervarya cancrivora which typically inhabits brackish water of mangrove forests of Southeast Asia. These two antimicrobial peptides, tigerinin-RC1 and -RC2 share significant structural similarity with tigerinins found in the skin of Indian frog, Hoplobatrachus tigerinus. cDNAs encoding tigerinin-RC1 and -RC2 were also cloned from the skin cDNA library of F. cancrivora. Tigerinin-RC precursors are composed of 71 amino acid residues including a signal peptide, acidic spacer peptide, which are very similar to other amphibian antimicrobial peptide precursors and mature tigerinin-RC. The current results confirmed that both amphibians inhabiting freshwater and brackish water share the same antimicrobial peptide family to exert innate defense. Furthermore, the current work was also the first report of precursor and cDNA cloning of the tigerinin antimicrobial peptide family.
关键词	Amphibian Antimicrobial Peptide Fejervarya Cancrivora Brackish Water Tigerinin
资助者	This work was supported by the Chinese National Natural Science Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R-088, KSCX2-YW-G-024), and the Ministry of Science (2008AA02Z133, 2008AA101001). ; This work was supported by the Chinese National Natural Science Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R-088, KSCX2-YW-G-024), and the Ministry of Science (2008AA02Z133, 2008AA101001). ; This work was supported by the Chinese National Natural Science Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R-088, KSCX2-YW-G-024), and the Ministry of Science (2008AA02Z133, 2008AA101001). ; This work was supported by the Chinese National Natural Science Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R-088, KSCX2-YW-G-024), and the Ministry of Science (2008AA02Z133, 2008AA101001).
收录类别	SCI
语种	英语
资助者	This work was supported by the Chinese National Natural Science Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R-088, KSCX2-YW-G-024), and the Ministry of Science (2008AA02Z133, 2008AA101001). ; This work was supported by the Chinese National Natural Science Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R-088, KSCX2-YW-G-024), and the Ministry of Science (2008AA02Z133, 2008AA101001). ; This work was supported by the Chinese National Natural Science Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R-088, KSCX2-YW-G-024), and the Ministry of Science (2008AA02Z133, 2008AA101001). ; This work was supported by the Chinese National Natural Science Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R-088, KSCX2-YW-G-024), and the Ministry of Science (2008AA02Z133, 2008AA101001).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2559
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai@mail.kiz.ac.cn
作者单位	1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China 3.College of Life Sciences, Hainan Normal University, Haikou 650223, Hainan, China 4.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
推荐引用方式 GB/T 7714	Song YZ,Lu Y,Wang LJ,et al. Purification, characterization and cloning of two novel tigerinin-like peptides from skin secretions of Fejervarya cancrivora[J]. PEPTIDES,2009,30(7):1228-1232.
APA	Song YZ.,Lu Y.,Wang LJ.,Yang HL.,Zhang KY.,...&rlai@mail.kiz.ac.cn.(2009).Purification, characterization and cloning of two novel tigerinin-like peptides from skin secretions of Fejervarya cancrivora.PEPTIDES,30(7),1228-1232.
MLA	Song YZ,et al."Purification, characterization and cloning of two novel tigerinin-like peptides from skin secretions of Fejervarya cancrivora".PEPTIDES 30.7(2009):1228-1232.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
20093071228.pdf（316KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文