Novel cathelicidin-derived antimicrobial peptides from Equus asinus

	Novel cathelicidin-derived antimicrobial peptides from Equus asinus
	Lu ZK 1; Wang YP 2,3; Zhai L 1; Che QL 3; Wang H 1; Du SY 1; Wang D 1; Feng FF 1,2; Liu JZ 1; Lai R3; Yu HN1,2; rlai@mail.kiz.ac.cn ; yuhaining@dlut.edu.cn
	2010
发表期刊	FEBS JOURNAL
ISSN	1742-464X
卷号	277 期号:10 页码:2329-2339
合作性质	其它
摘要	In the present study, EA-CATH1 and EA-CATH2 were identified from a constructed lung cDNA library of donkey (Equus asinus) as members of cathelicidin-derived antimicrobial peptides, using a nested PCR-based cloning strategy. Composed of 25 and 26 residues, respectively, EA-CATH1 and EA-CATH2 are smaller than most other cathelicidins and have no sequence homology to other cathelicidins identified to date. Chemically synthesized EA-CATH1 exerted potent antimicrobial activity against most of the 32 strains of bacteria and fungi tested, especially the clinically isolated drug-resistant strains, and minimal inhibitory concentration values against Gram-positive bacteria were mostly in the range of 0.3-2.4 mu g center dot mL-1. EA-CATH1 showed an extraordinary serum stability and no haemolytic activity against human erythrocytes in a dose up to 20 mu g center dot mL-1. CD spectra showed that EA-CATH1 mainly adopts an alpha-helical conformation in a 50% trifluoroethanol/water solution, but a random coil in aqueous solution. Scanning electron microscope observations of Staphylococcus aureus (ATCC2592) treated with EA-CATH1 demonstrated that EA-CATH could cause rapid disruption of the bacterial membrane, and in turn lead to cell lysis. This might explain the much faster killing kinetics of EA-CATH1 than conventional antibiotics revealed by killing kinetics data. In the presence of CaCl(2), EA-CATH1 exerted haemagglutination activity, which might potentiate an inhibition against the bacterial polyprotein interaction with the host erythrocyte surface, thereby possibly restricting bacterial colonization and spread.
关键词	Cathelicidin Equus Asinus Function Gene Cloning Peptide Identification
资助者	This work was supported by grants from the Chinese National Natural Science Foundation (30900240). ; This work was supported by grants from the Chinese National Natural Science Foundation (30900240). ; This work was supported by grants from the Chinese National Natural Science Foundation (30900240). ; This work was supported by grants from the Chinese National Natural Science Foundation (30900240).
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收录类别	SCI
语种	英语
资助者	This work was supported by grants from the Chinese National Natural Science Foundation (30900240). ; This work was supported by grants from the Chinese National Natural Science Foundation (30900240). ; This work was supported by grants from the Chinese National Natural Science Foundation (30900240). ; This work was supported by grants from the Chinese National Natural Science Foundation (30900240).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2573
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai@mail.kiz.ac.cn; yuhaining@dlut.edu.cn
作者单位	1.College of Life Sciences, Hebei Normal University, Shijiazhuang, China 2.School of Life Science and Biotechnology, Dalian University of Technology, China 3.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China
推荐引用方式 GB/T 7714	Lu ZK,Wang YP,Zhai L,et al. Novel cathelicidin-derived antimicrobial peptides from Equus asinus[J]. FEBS JOURNAL,2010,277(10):2329-2339.
APA	Lu ZK.,Wang YP.,Zhai L.,Che QL.,Wang H.,...&yuhaining@dlut.edu.cn.(2010).Novel cathelicidin-derived antimicrobial peptides from Equus asinus.FEBS JOURNAL,277(10),2329-2339.
MLA	Lu ZK,et al."Novel cathelicidin-derived antimicrobial peptides from Equus asinus".FEBS JOURNAL 277.10(2010):2329-2339.

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