Cloning and characterization of the first amphibian bradykinin gene

	Cloning and characterization of the first amphibian bradykinin gene
	Chen MD 1; Che QL 1; Wang X 1; Li JX 2; Yang HL 2,3; Li DS 2; Zhang KY 1; Lai R*1,2; rlai72@njau.edu.cn
	2010
发表期刊	BIOCHIMIE
ISSN	0300-9084
卷号	92 期号:3 页码:226-231
合作性质	其它
摘要	More than ten bradykinin-related peptides and their cDNAs; have been identified from amphibians, but their genes are unknown. In present study, four cDNAs encoding one, two, four and six copies of bradykinin-related peptides were cloned from the frog (Odorrana grahami) skin cDNA library, respectively. Three bradykinin-related peptides (bradykinin, Thr6-bradykinin, Leu5Thr6-bradykinin) were deduced from these four cDNA sequences. Based on the cDNA sequence, the gene sequence encoding an amphibian bradykinin-related peptide from O. grahami was determined. It is composed of 7481 base pairs including two exons and two introns. The first exon codes signal peptide and the second exon codes acidic spacer peptide and Thr6-bradykinin. The promoter region of the bradykinin gene contains several putative recognition sites for nuclear factors, such as SRY, GATA-1, LYF-1, DeltaE, CDXA, NKX-2.5, MIF1 and S8. The current work may facilitate to understand the regulation and possible functions of amphibian skin bradykinin-related peptides.
关键词	Amphibian Bradykinin Gene Skin
资助者	This Study was supported by grants from the 973 program (2009CB941703, 2006CB504002), the Chinese Natural Science Funds (30630030), and the Program of Changiiang Scholars and Innovative Research Team in University (IRT0631). ; This Study was supported by grants from the 973 program (2009CB941703, 2006CB504002), the Chinese Natural Science Funds (30630030), and the Program of Changiiang Scholars and Innovative Research Team in University (IRT0631). ; This Study was supported by grants from the 973 program (2009CB941703, 2006CB504002), the Chinese Natural Science Funds (30630030), and the Program of Changiiang Scholars and Innovative Research Team in University (IRT0631). ; This Study was supported by grants from the 973 program (2009CB941703, 2006CB504002), the Chinese Natural Science Funds (30630030), and the Program of Changiiang Scholars and Innovative Research Team in University (IRT0631).
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收录类别	SCI
语种	英语
资助者	This Study was supported by grants from the 973 program (2009CB941703, 2006CB504002), the Chinese Natural Science Funds (30630030), and the Program of Changiiang Scholars and Innovative Research Team in University (IRT0631). ; This Study was supported by grants from the 973 program (2009CB941703, 2006CB504002), the Chinese Natural Science Funds (30630030), and the Program of Changiiang Scholars and Innovative Research Team in University (IRT0631). ; This Study was supported by grants from the 973 program (2009CB941703, 2006CB504002), the Chinese Natural Science Funds (30630030), and the Program of Changiiang Scholars and Innovative Research Team in University (IRT0631). ; This Study was supported by grants from the 973 program (2009CB941703, 2006CB504002), the Chinese Natural Science Funds (30630030), and the Program of Changiiang Scholars and Innovative Research Team in University (IRT0631).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2577
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai72@njau.edu.cn
作者单位	1.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 3.Graduate School of the Chinese Academy of Sciences, Beijing100009, China
推荐引用方式 GB/T 7714	Chen MD,Che QL,Wang X,et al. Cloning and characterization of the first amphibian bradykinin gene[J]. BIOCHIMIE,2010,92(3):226-231.
APA	Chen MD.,Che QL.,Wang X.,Li JX.,Yang HL.,...&rlai72@njau.edu.cn.(2010).Cloning and characterization of the first amphibian bradykinin gene.BIOCHIMIE,92(3),226-231.
MLA	Chen MD,et al."Cloning and characterization of the first amphibian bradykinin gene".BIOCHIMIE 92.3(2010):226-231.

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