Stejnulxin, a novel snake C-type lectin-like protein from Trimeresurus stejnegeri venom is a potent platelet agonist acting specifically via GPVl

	Stejnulxin, a novel snake C-type lectin-like protein from Trimeresurus stejnegeri venom is a potent platelet agonist acting specifically via GPVl
	Lee WH 1; Du XY 2; Lu QM 1,2; Clemetson KJ 2; Zhang Y*1; zhangy@mail.kiz.ac.en
	2003
发表期刊	THROMBOSIS AND HAEMOSTASIS
ISSN	0340-6245
卷号	90 期号:4 页码:662-671
摘要	Stejnulxin, a novel snake C-type lectin-like protein with potent platelet activating activity, was purified and characterized from Trimeresurus stejnegeri venom. Under non-reducing conditions, it migrated on a SDS-polyacrylamide gel with an apparent molecular mass of 120 kDa. On reduction, it separated into three polypeptide subunits with apparent molecular masses of 16 kDa (alpha), 20 kDa (beta1) and 22 kDa (beta2), respectively. The complete amino acid sequences of its subunits were deduced from cloned cDNAs. The N-terminal sequencing and cDNA cloning indicated that beta1 and beta2 subunits of stejnulxin have identical amino acid sequences and each contains two N-glycosylation sites. Accordingly, the molecular mass difference between beta1 and beta2 is caused by glycosylation heterogenity. The subunit amino acid sequences of stejnulxin are similar to those of convulxin, with sequence identities of 52.6% and 66.4% for the alpha and beta, respectively. Stejnulxin induced human platelet aggregation in a dose-dependent manner. Antibodies against alphaIIbbeta3 inhibited the aggregation response to stejnulxin, indicating that activation of alphaIIbbeta3 and binding of fibrinogen are involved in stejnulxin-induced platelet aggregation. Antibodies against GPIbalpha or alpha2beta1 as well as echicetin or rhodocetin had no significant effect on stejnulxin-induced platelet aggregation. However, platelet activation induced by stejnulxin was blocked by anti-GPVI antibodies. In addition, stejnulxin induced a tyrosine phosphorylation profile in platelets that resembled that produced by convulxin. Biotinylated stejnulxin bound specifically to platelet membrane GPVI.
关键词	Stejnulxin Platelet Aggregation Gpvi Tyrosine Phosphorylatio
资助者	This work was supported by grants from “STZ98-3-01”, “Western Light Project” and “Tenth Five Year Plan” pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (2001C0061M). ; This work was supported by grants from “STZ98-3-01”, “Western Light Project” and “Tenth Five Year Plan” pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (2001C0061M). ; This work was supported by grants from “STZ98-3-01”, “Western Light Project” and “Tenth Five Year Plan” pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (2001C0061M). ; This work was supported by grants from “STZ98-3-01”, “Western Light Project” and “Tenth Five Year Plan” pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (2001C0061M).
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收录类别	SCI
语种	英语
资助者	This work was supported by grants from “STZ98-3-01”, “Western Light Project” and “Tenth Five Year Plan” pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (2001C0061M). ; This work was supported by grants from “STZ98-3-01”, “Western Light Project” and “Tenth Five Year Plan” pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (2001C0061M). ; This work was supported by grants from “STZ98-3-01”, “Western Light Project” and “Tenth Five Year Plan” pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (2001C0061M). ; This work was supported by grants from “STZ98-3-01”, “Western Light Project” and “Tenth Five Year Plan” pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (2001C0061M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2647
专题	科研部门_生物毒素与人类疾病（张云）科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	zhangy@mail.kiz.ac.en
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology,The Chinese Academy of Sciences, Kunming,Yunnan, P. R. China 2.Theodor Kocher Institute, University of Berne, Berne, Switzerland
推荐引用方式 GB/T 7714	Lee WH,Du XY,Lu QM,et al. Stejnulxin, a novel snake C-type lectin-like protein from Trimeresurus stejnegeri venom is a potent platelet agonist acting specifically via GPVl[J]. THROMBOSIS AND HAEMOSTASIS,2003,90(4):662-671.
APA	Lee WH,Du XY,Lu QM,Clemetson KJ,Zhang Y*,&zhangy@mail.kiz.ac.en.(2003).Stejnulxin, a novel snake C-type lectin-like protein from Trimeresurus stejnegeri venom is a potent platelet agonist acting specifically via GPVl.THROMBOSIS AND HAEMOSTASIS,90(4),662-671.
MLA	Lee WH,et al."Stejnulxin, a novel snake C-type lectin-like protein from Trimeresurus stejnegeri venom is a potent platelet agonist acting specifically via GPVl".THROMBOSIS AND HAEMOSTASIS 90.4(2003):662-671.

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