Molecular cloning and characterization of a platelet glycoprotein Ib-binding protein from the venom of Trimeresurus stejnegeri

	Molecular cloning and characterization of a platelet glycoprotein Ib-binding protein from the venom of Trimeresurus stejnegeri
	Lee WH 1,2; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2003
发表期刊	TOXICON
ISSN	0041-0101
卷号	41 期号:7 页码:885-892
合作性质	其它
摘要	A platelet glycoprotein Ib-binding protein, termed TSV-GPIb-BP, was isolated from the venom of Trimeresurus stejnegeri. On SDS-polyacrylamide gel electrophoresis, TSV-GPIb-BP showed a single band with an apparent molecular weight of 28,000 and two distinct bands with apparent molecular weights of 16,000 and 15,000 under non-reducing and reducing conditions, respectively. cDNA clones containing the coding sequences for both TSV-GPIb-BP subunits were isolated and sequenced. The deduced amino acid sequences of TSV-GPIb-BP subunits were confirmed by N-terminal protein sequencing and trypsin-digested peptide mass fingerprinting. Interestingly, the alpha subunit of TSV-GPIb-BP is identical to that of alboaggregin-B, and the sequence identity of their beta subunits is 94.3%. TSV-GPIb-BP inhibited ristocetin-induced human platelet agglutination in platelet-rich plasma under lower dosages (<5 microg/ml). On the other hand, it directly aggregated washed human platelets in the absence of additional Ca2+ or any other cofactors under higher dosages (>5 microg/ml). This platelet aggregation activity was dose-dependently inhibited by specific GPIbalpha antibodies, but not by those antibodies against platelet GPIa, GPIIa, GPIIb and GPIIIa.
关键词	Snake Venom GlycoProtein Ib-binding Protein Trimeresurus Stejnegeri Cdna Sequence
资助者	This work was supported by the grants of ‘Western Light Project’ and ‘Tenth 5 Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth 5 Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth 5 Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth 5 Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M).
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收录类别	SCI
语种	英语
资助者	This work was supported by the grants of ‘Western Light Project’ and ‘Tenth 5 Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth 5 Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth 5 Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth 5 Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2655
专题	科研部门_生物毒素与人类疾病（张云）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, People’s Republic of China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, People’s Republic of China
推荐引用方式 GB/T 7714	Lee WH,Zhang Y*,zhangy@mail.kiz.ac.cn. Molecular cloning and characterization of a platelet glycoprotein Ib-binding protein from the venom of Trimeresurus stejnegeri[J]. TOXICON,2003,41(7):885-892.
APA	Lee WH,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2003).Molecular cloning and characterization of a platelet glycoprotein Ib-binding protein from the venom of Trimeresurus stejnegeri.TOXICON,41(7),885-892.
MLA	Lee WH,et al."Molecular cloning and characterization of a platelet glycoprotein Ib-binding protein from the venom of Trimeresurus stejnegeri".TOXICON 41.7(2003):885-892.

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