TMVA, a snake C-type lectin-like protein from Trimeresurus mucrosquamatus venom, activates platelet via GPIb

	TMVA, a snake C-type lectin-like protein from Trimeresurus mucrosquamatus venom, activates platelet via GPIb
	Tai H 1,2,3; Wei Q 1; Jin Y 1; Su M 3; Song JX 3; Zhou XD 1,2; Ouyang HM 3; Wang WY 1; Xiong YL*1; Zhang Y 1; toxinlab@mail.kiz.ac.cn
	2004
发表期刊	TOXICON
ISSN	0041-0101
卷号	44 期号:6 页码:649-656
合作性质	其它
摘要	TMVA is a C-type lectin-like protein with potent platelet activating activity from Trimeresurus mucrosquamatus venom. In the absence of von Willebrand factor (vWF), TMVA dose-dependently induced aggregation of washed platelets. Anti-GP Ib monoclonal antibodies (mAbs), HIP1, specifically inhibited TMVA-induced aggregation in a dose-dependent manner. The aggregation was also inhibited by mAb P2 (an anti-GP IIb mAb). Flow cytometric analysis revealed that FITC-TMVA bound to human formalin-fixed platelets in a saturable manner, and its binding was specifically blocked by HIP1 in a dose-dependent manner. Flow cytometric analysis showed that TMVA did not bind to platelet GPIX, GPIIb, GPIIIa, GPIa, GPIIa and GPIV. Moreover, the platelet aggregation induced by TMVA was partially inhibited when platelet was pretreated with mocarhagin, a snake venom protease that specifically cleaves human GPIb. These results suggest that TMVA is a strong platelet agonist via GPIb and it might have multiple functional binding-sites on GPIb molecule or on other unknown receptor.
关键词	Platelet Aggregation Tmva Gpib Mocarhagin
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收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2659
专题	科研部门_生物毒素与人类疾病（张云）其他
通讯作者	toxinlab@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, People’s Republic of China 2.Graduate School of the Chinese Academy of Sciences, People’s Republic of China 3.The First People’s Hospital of Yunnan Province, Kunming, People’s Republic of China
推荐引用方式 GB/T 7714	Tai H,Wei Q,Jin Y,et al. TMVA, a snake C-type lectin-like protein from Trimeresurus mucrosquamatus venom, activates platelet via GPIb[J]. TOXICON,2004,44(6):649-656.
APA	Tai H.,Wei Q.,Jin Y.,Su M.,Song JX.,...&toxinlab@mail.kiz.ac.cn.(2004).TMVA, a snake C-type lectin-like protein from Trimeresurus mucrosquamatus venom, activates platelet via GPIb.TOXICON,44(6),649-656.
MLA	Tai H,et al."TMVA, a snake C-type lectin-like protein from Trimeresurus mucrosquamatus venom, activates platelet via GPIb".TOXICON 44.6(2004):649-656.

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