Cloning and purification of alpha-neurotoxins from king cobra (Ophiophagus hannah)

	Cloning and purification of alpha-neurotoxins from king cobra (Ophiophagus hannah)
	He YY 1,2; Lee WH 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2004
发表期刊	TOXICON
ISSN	0041-0101
卷号	44 期号:3 页码:295-303
合作性质	其它
摘要	Thirteen complete and three partial cDNA sequences were cloned from the constructed king cobra (Ophiophagus hannah) venom gland cDNA library. Phylogenetic analysis of nucleotide sequences of king cobra with those from other snake venoms revealed that obtained cDNAs are highly homologous to snake venom alpha-neurotoxins. Alignment of deduced mature peptide sequences of the obtained clones with those of other reported alpha-neurotoxins from the king cobra venom indicates that our obtained 16 clones belong to long-chain neurotoxins (seven), short-chain neurotoxins (seven), weak toxin (one) and variant (one), respectively. Up to now, two out of 16 newly cloned king cobra alpha-neurotoxins have identical amino acid sequences with CM-11 and Oh-6A/6B, which have been characterized from the same venom. Furthermore, five long-chain alpha-neurotoxins and two short-chain alpha-neurotoxins were purified from crude venom and their N-terminal amino acid sequences were determined. The cDNAs encoding the putative precursors of the purified native peptide were also determined based on the N-terminal amino acid sequencing. The purified alpha-neurotoxins showed different lethal activities on mice.
关键词	Snake Venom A-neurotoxin Ophiophagus Hannah Cdna Sequence
资助者	This work was supported by grants from Yunnan Science and Technology Commission (2001C0062M, 2001C0061M, 2003C0066M); ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Year Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195). ; This work was supported by grants from Yunnan Science and Technology Commission (2001C0062M, 2001C0061M, 2003C0066M); ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Year Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195). ; This work was supported by grants from Yunnan Science and Technology Commission (2001C0062M, 2001C0061M, 2003C0066M); ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Year Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195). ; This work was supported by grants from Yunnan Science and Technology Commission (2001C0062M, 2001C0061M, 2003C0066M); ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Year Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195).
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收录类别	SCI
语种	英语
资助者	This work was supported by grants from Yunnan Science and Technology Commission (2001C0062M, 2001C0061M, 2003C0066M); ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Year Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195). ; This work was supported by grants from Yunnan Science and Technology Commission (2001C0062M, 2001C0061M, 2003C0066M); ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Year Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195). ; This work was supported by grants from Yunnan Science and Technology Commission (2001C0062M, 2001C0061M, 2003C0066M); ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Year Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195). ; This work was supported by grants from Yunnan Science and Technology Commission (2001C0062M, 2001C0061M, 2003C0066M); ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Year Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2661
专题	科研部门_生物毒素与人类疾病（张云）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming 650223, Yunnan, China 2.China Three Gorge University, Yichang, 443002 Hubei, China
推荐引用方式 GB/T 7714	He YY,Lee WH,Zhang Y*,et al. Cloning and purification of alpha-neurotoxins from king cobra (Ophiophagus hannah)[J]. TOXICON,2004,44(3):295-303.
APA	He YY,Lee WH,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2004).Cloning and purification of alpha-neurotoxins from king cobra (Ophiophagus hannah).TOXICON,44(3),295-303.
MLA	He YY,et al."Cloning and purification of alpha-neurotoxins from king cobra (Ophiophagus hannah)".TOXICON 44.3(2004):295-303.

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