Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima

	Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima
	Shen JH 1,2; Liu SB 1; Zhang YX 1; Jin Y 1; Lee WH 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2005
发表期刊	REGULATORY PEPTIDES
ISSN	0167-0115
卷号	132 期号:1-3 页码:102-106
摘要	Amphibian skin is a rich resource of bioactive peptides like proline-rich bombesin from frog Bombina maxima. A novel cDNA clone encoding a precursor protein that comprises proline-rich bombesin and a novel peptide, designated as bombestatin, was isolated from a skin cDNA library of B. maxima. The predicted primary structure of the novel peptide is WEVLLNVALIRLELLSCRSSKDQDQKESCGMHSW, in which two cysteines form a disulfide bond. A BLAST search of databases did not detect sequences with significant similarity. Bombestatin possesses dose-dependent contractile activity on rat stomach strips. The differences between cDNAs encoding PR-bombesin plus bombestatin and PR-bombesin alone are due to fragment insertions located in 3'-coding region and 3'-untranslational region, respectively.
关键词	Amphibian Bombesin Bombestatin Bombina Maxima Cdna Sequence
资助者	This work was supported by ‘‘Western Light Project’’ from The Chinese Academy of Sciences to Dr. W-H. Lee and Dr. Y. Zhang; and grants from National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by ‘‘Western Light Project’’ from The Chinese Academy of Sciences to Dr. W-H. Lee and Dr. Y. Zhang; and grants from National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by ‘‘Western Light Project’’ from The Chinese Academy of Sciences to Dr. W-H. Lee and Dr. Y. Zhang; and grants from National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by ‘‘Western Light Project’’ from The Chinese Academy of Sciences to Dr. W-H. Lee and Dr. Y. Zhang; and grants from National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M).
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收录类别	SCI
语种	英语
资助者	This work was supported by ‘‘Western Light Project’’ from The Chinese Academy of Sciences to Dr. W-H. Lee and Dr. Y. Zhang; and grants from National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by ‘‘Western Light Project’’ from The Chinese Academy of Sciences to Dr. W-H. Lee and Dr. Y. Zhang; and grants from National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by ‘‘Western Light Project’’ from The Chinese Academy of Sciences to Dr. W-H. Lee and Dr. Y. Zhang; and grants from National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by ‘‘Western Light Project’’ from The Chinese Academy of Sciences to Dr. W-H. Lee and Dr. Y. Zhang; and grants from National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2663
专题	科研部门_生物毒素与人类疾病（张云）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 2.The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China
推荐引用方式 GB/T 7714	Shen JH,Liu SB,Zhang YX,et al. Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima[J]. REGULATORY PEPTIDES,2005,132(1-3):102-106.
APA	Shen JH.,Liu SB.,Zhang YX.,Jin Y.,Lee WH.,...&zhangy@mail.kiz.ac.cn.(2005).Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima.REGULATORY PEPTIDES,132(1-3),102-106.
MLA	Shen JH,et al."Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima".REGULATORY PEPTIDES 132.1-3(2005):102-106.

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