Isolation and preliminary characterization of a 22-kDa protein with trypsin inhibitory activity from toad Bufo andrewsi skin

	Isolation and preliminary characterization of a 22-kDa protein with trypsin inhibitory activity from toad Bufo andrewsi skin
	Zhao Y 1,2; Jin Y 1; Lee WH 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2005
发表期刊	TOXICON
ISSN	0041-0101
卷号	46 期号:3 页码:277-281
合作性质	其它
摘要	A novel trypsin inhibitor termed BATI was purified to homogeneity from the skin extracts of toad Bufo andrewsi by successive ion-exchange, gel-filtration and reverse-phase chromatography. BATI is basic single chain glycoprotein, with apparent molecular weight of 22 kDa in SDS-PAGE. BATI is a thermal stable competitive inhibitor and effectively inhibits trypsin's catalytic activity on peptide substrate with the inhibitor constant (K(i)) value of 14 nM and shows no inhibitory effect on chymotrypsin, thrombin and elastase. The N-terminal sequence of BATI is EKDSITD, which shows no similarity with other known trypsin inhibitors.
关键词	Trypsin Inhibitor Bufo Andrewsi Skin
资助者	This work was supported by the grants of ‘Western Light’ Projects and grants from the National Natural Science Foundation (30170195, 30470380). ; This work was supported by the grants of ‘Western Light’ Projects and grants from the National Natural Science Foundation (30170195, 30470380). ; This work was supported by the grants of ‘Western Light’ Projects and grants from the National Natural Science Foundation (30170195, 30470380). ; This work was supported by the grants of ‘Western Light’ Projects and grants from the National Natural Science Foundation (30170195, 30470380).
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收录类别	SCI
语种	英语
资助者	This work was supported by the grants of ‘Western Light’ Projects and grants from the National Natural Science Foundation (30170195, 30470380). ; This work was supported by the grants of ‘Western Light’ Projects and grants from the National Natural Science Foundation (30170195, 30470380). ; This work was supported by the grants of ‘Western Light’ Projects and grants from the National Natural Science Foundation (30170195, 30470380). ; This work was supported by the grants of ‘Western Light’ Projects and grants from the National Natural Science Foundation (30170195, 30470380).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2671
专题	科研部门_生物毒素与人类疾病（张云）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, People’s Republic of China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, People’s Republic of China
推荐引用方式 GB/T 7714	Zhao Y,Jin Y,Lee WH,et al. Isolation and preliminary characterization of a 22-kDa protein with trypsin inhibitory activity from toad Bufo andrewsi skin[J]. TOXICON,2005,46(3):277-281.
APA	Zhao Y,Jin Y,Lee WH,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2005).Isolation and preliminary characterization of a 22-kDa protein with trypsin inhibitory activity from toad Bufo andrewsi skin.TOXICON,46(3),277-281.
MLA	Zhao Y,et al."Isolation and preliminary characterization of a 22-kDa protein with trypsin inhibitory activity from toad Bufo andrewsi skin".TOXICON 46.3(2005):277-281.

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