Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima

	Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima
	Lee WH 1; Liu SB 1,2; Shen JH 1,3; Jin Y 1; Lai R 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2005
发表期刊	REGULATORY PEPTIDES
ISSN	0167-0115
卷号	129 期号:1-3 页码:43-47
合作性质	其它
摘要	Amphibian skin contains rich neuropeptides. In the present study, a novel neuromedin U (NmU) analog was isolated from skin secretions of Chinese red belly toad Bombina maxima. Being 17-amino acids long, its primary structure was established as DSSGIVGRPFFLFRPRN-NH2, in which the C-terminal 8-residue segment (FFLFRPRN) is the same as that of rat NmU, while the N-terminal part DSSGIVGRP shows a great sequence variation compared with those of NmU peptides from different resources. The peptide, named Bm-NmU-17, was found to elicit concentration-dependent contractile effects on smooth muscle of rat uterus horns. The cDNA structure of the peptide, as obtained by a 3'-RACE strategy and subsequently cloning from a skin cDNA library, was found to contain a coding region of 438 nucleotides. The encoded precursor is composed of 145 amino acids with a single copy of Bm-NmU-17 located towards the C-terminus. The sequence of the peptide is preceded by a dibasic site (Lys-Arg) and followed by the sequence of Gly-Arg-Lys, providing the sites of cleavage and releasing of the mature peptide.
关键词	Neuropeptide Neuromedin u Amphibian Bombina Maxima Cdna Sequence
资助者	This work was supported by the grants of bWestern Light ProjectQ and bTenth Five Years PlanQ pre-research project from The Chinese Academy of Sciences; and grants from National Natural Science Foundation (30170195, 30200044, 30470380) and Yunnan Science and Technology Commis- sion (2001C0061M, 2001C0062M and 2003C0066M). ; This work was supported by the grants of bWestern Light ProjectQ and bTenth Five Years PlanQ pre-research project from The Chinese Academy of Sciences; and grants from National Natural Science Foundation (30170195, 30200044, 30470380) and Yunnan Science and Technology Commis- sion (2001C0061M, 2001C0062M and 2003C0066M). ; This work was supported by the grants of bWestern Light ProjectQ and bTenth Five Years PlanQ pre-research project from The Chinese Academy of Sciences; and grants from National Natural Science Foundation (30170195, 30200044, 30470380) and Yunnan Science and Technology Commis- sion (2001C0061M, 2001C0062M and 2003C0066M). ; This work was supported by the grants of bWestern Light ProjectQ and bTenth Five Years PlanQ pre-research project from The Chinese Academy of Sciences; and grants from National Natural Science Foundation (30170195, 30200044, 30470380) and Yunnan Science and Technology Commis- sion (2001C0061M, 2001C0062M and 2003C0066M).
URL	查看原文
收录类别	SCI
语种	英语
资助者	This work was supported by the grants of bWestern Light ProjectQ and bTenth Five Years PlanQ pre-research project from The Chinese Academy of Sciences; and grants from National Natural Science Foundation (30170195, 30200044, 30470380) and Yunnan Science and Technology Commis- sion (2001C0061M, 2001C0062M and 2003C0066M). ; This work was supported by the grants of bWestern Light ProjectQ and bTenth Five Years PlanQ pre-research project from The Chinese Academy of Sciences; and grants from National Natural Science Foundation (30170195, 30200044, 30470380) and Yunnan Science and Technology Commis- sion (2001C0061M, 2001C0062M and 2003C0066M). ; This work was supported by the grants of bWestern Light ProjectQ and bTenth Five Years PlanQ pre-research project from The Chinese Academy of Sciences; and grants from National Natural Science Foundation (30170195, 30200044, 30470380) and Yunnan Science and Technology Commis- sion (2001C0061M, 2001C0062M and 2003C0066M). ; This work was supported by the grants of bWestern Light ProjectQ and bTenth Five Years PlanQ pre-research project from The Chinese Academy of Sciences; and grants from National Natural Science Foundation (30170195, 30200044, 30470380) and Yunnan Science and Technology Commis- sion (2001C0061M, 2001C0062M and 2003C0066M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2675
专题	科研部门_生物毒素与人类疾病（张云）科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming 650223, Yunnan, China 2.Graduate School of The Chinese Academy of Sciences, Beijing 100039, China 3.The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China
推荐引用方式 GB/T 7714	Lee WH,Liu SB,Shen JH,et al. Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima[J]. REGULATORY PEPTIDES,2005,129(1-3):43-47.
APA	Lee WH.,Liu SB.,Shen JH.,Jin Y.,Lai R.,...&zhangy@mail.kiz.ac.cn.(2005).Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima.REGULATORY PEPTIDES,129(1-3),43-47.
MLA	Lee WH,et al."Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima".REGULATORY PEPTIDES 129.1-3(2005):43-47.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
200512943.pdf（125KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文