A snake venom metalloproteinase that inhibited cell proliferation and induced morphological changes of ECV304 cells

	A snake venom metalloproteinase that inhibited cell proliferation and induced morphological changes of ECV304 cells
	Wan SG 1,2; Jin Y 1; Lee WH 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2006
发表期刊	TOXICON
ISSN	0041-0101
卷号	47 期号:4 页码:480-489
摘要	TSV-DM, a basic metalloproteinase with a molecular weight of 110kDa, was purified from Trimeresurus stejnegeri venom. TSV-DM degraded the Aalpha chain of fibrinogen more rapidly than the Bbeta chain in a dose dependent manner. The cDNA of TSV-DM encoded a polypeptide of 622 amino acid residues, which comprises a signal peptide, proprotein, metalloproteinase domain, spacer, disintegrin-like domain and cysteine-rich domain. The protein sequence deduced from cDNA was confirmed by peptide mass fingerprinting analysis. It is highly homologous to the members of subclass P-IIIb snake venom metalloproteinase, which comprises vascular apoptosis-inducing proteins. TSV-DM inhibited cell proliferation and induced cell morphologic changes transiently of ECV304 cells. However, DNA fragmentation and DNA content analysis demonstrated that this metalloproteinase could not induce ECV304 cells apoptosis.
关键词	Metalloproteinase Apoptosis Vascular Endothelial Cell Snake Venom
资助者	This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30170195, 30470380 and 30570359) and Yunnan Science and Technology Commission (2003C0066M and 2005PY01-23). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30170195, 30470380 and 30570359) and Yunnan Science and Technology Commission (2003C0066M and 2005PY01-23). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30170195, 30470380 and 30570359) and Yunnan Science and Technology Commission (2003C0066M and 2005PY01-23). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30170195, 30470380 and 30570359) and Yunnan Science and Technology Commission (2003C0066M and 2005PY01-23).
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收录类别	SCI
语种	英语
资助者	This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30170195, 30470380 and 30570359) and Yunnan Science and Technology Commission (2003C0066M and 2005PY01-23). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30170195, 30470380 and 30570359) and Yunnan Science and Technology Commission (2003C0066M and 2005PY01-23). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30170195, 30470380 and 30570359) and Yunnan Science and Technology Commission (2003C0066M and 2005PY01-23). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30170195, 30470380 and 30570359) and Yunnan Science and Technology Commission (2003C0066M and 2005PY01-23).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2691
专题	科研部门_生物毒素与人类疾病（张云）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Wan SG,Jin Y,Lee WH,et al. A snake venom metalloproteinase that inhibited cell proliferation and induced morphological changes of ECV304 cells[J]. TOXICON,2006,47(4):480-489.
APA	Wan SG,Jin Y,Lee WH,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2006).A snake venom metalloproteinase that inhibited cell proliferation and induced morphological changes of ECV304 cells.TOXICON,47(4),480-489.
MLA	Wan SG,et al."A snake venom metalloproteinase that inhibited cell proliferation and induced morphological changes of ECV304 cells".TOXICON 47.4(2006):480-489.

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