Characterization and molecular cloning of dabocetin, a potent antiplatelet C-type lectin-like protein from Daboia russellii siamensis venom

	Characterization and molecular cloning of dabocetin, a potent antiplatelet C-type lectin-like protein from Daboia russellii siamensis venom
	Zhong SR 1,2; Jin Y 1; Wu JB 1,2; Chen RQ 1,2; Jia YH 3; Wang WY 1; Xiong YL*1; Zhang Y 1; zhongshurong@hotmail.com
	2006
发表期刊	TOXICON
ISSN	0041-0101
卷号	47 期号:1 页码:104-112
合作性质	其它
摘要	A novel C-type lectin-like protein, dabocetin, was purified from Daboia russellii siamensis venom. On SDS-polyacrylamide gel electrophoresis, it showed a single band with an apparent molecular weight of 28 kDa and two distinct bands with the apparent molecular weights of 15.0 kDa and 14.5 kDa under non-reducing and reducing conditions, respectively. cDNA clones containing the coding sequences for dabocetin alpha and beta subunits were isolated and sequenced. The deduced protein sequences of both subunits were confirmed by N-terminal amino acid sequencing and trypsin-digested peptide mass fingerprinting. Dabocetin did not induce platelet aggregation in platelet-rich plasma. It also had little effect on the platelet aggregation induced by ADP, TMVA or stejnulxin. Whereas, dabocetin inhibited ristocetin-induced platelet agglutination in platelet-rich plasma in a dose-dependent manner with an IC50 value of 0.35 microM. Flow cytometry analysis showed that dabocetin significantly inhibited mAb SZ2 binding to platelet membrane glycoprotein Ib alpha, indicating that platelet membrane glycoprotein Ib is involved in the inhibitory effect of dabocetin on ristocetin-induced platelet agglutination.
关键词	Sv-ctlp Platelet Aggregation/agglutination Cdna Sequence Daboia Russellii Siamensis Venom
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收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2697
专题	科研部门_生物毒素与人类疾病（张云）其他
通讯作者	zhongshurong@hotmail.com
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3.Faculty of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
推荐引用方式 GB/T 7714	Zhong SR,Jin Y,Wu JB,et al. Characterization and molecular cloning of dabocetin, a potent antiplatelet C-type lectin-like protein from Daboia russellii siamensis venom[J]. TOXICON,2006,47(1):104-112.
APA	Zhong SR.,Jin Y.,Wu JB.,Chen RQ.,Jia YH.,...&zhongshurong@hotmail.com.(2006).Characterization and molecular cloning of dabocetin, a potent antiplatelet C-type lectin-like protein from Daboia russellii siamensis venom.TOXICON,47(1),104-112.
MLA	Zhong SR,et al."Characterization and molecular cloning of dabocetin, a potent antiplatelet C-type lectin-like protein from Daboia russellii siamensis venom".TOXICON 47.1(2006):104-112.

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